Nce it has been demonstrated that transcription from the PP5 promoter could be reduced through inhibiting estrogen receptor (ER) (Urban et al., 2001). Therefore, we established J-Lat 9.2 and control J-Lat 8.4 cell lines that stably express miRNA-adapted shRNA (shRNAmir) against the ER mRNA (J-Lat 9.2 shER and J-Lat 8.4 shER, respectively). In both J-Lat shER cell lines, the ER levels were decrease
Me transcription through the provirus, since slight enrichment of elongating RNAPII in the HIV coding region of transcriptionally silent J-Lat 9.2 cells was observed using ChIPqPCR assay. However, transcription originating from the host promoter, ignoring pA sites in both LTRs and consequently splicing out the provirus together with the host intron (Han et al., 2004) is most likely less frequent t
Vels of viral particles, we speculate that average levels of host-viral chimeric transcripts in these cells exceed those in the two J-Lat cell lines. These results thus suggest that TI plays a key role in primary CD4+ T cells. In J-Lat 9.2 and 15.4 cells, the host-viral chimeric transcripts terminated at the pA site in the 5'LTR, but transcripts initiating at the host promoter and terminating at t
Y i is given by:Verguet Cost Effectiveness and Resource Allocation 2013, 11:1 http://www.resource-allocation.com/content/11/1/Page 3 ofTable 1 Cost parameters for the male circumcision interventionIntervention Initial investment per circumcision facility CF Initial training per circumciser CT Salary of each circumciser CC Salary of health care workers/counselors per circumciser Cost of supplies pe
M et al.Author ManuscriptAuthor ManuscriptAuthor ManuscriptGautam et al.DFZWPage13.11.11.10.9.6.2.2.1.1.0.0.0.DFZV16.13.14.12.12.11.10.10.0.ACKNOWLEDGEMENTSWe thank R. Plishka, A. Peach and T. Lewis for determining plasma viral RNA loads and K. Rice, R. Engel, R. Petros and S. Fong for diligently assisting in the maintenance of animals and assisting with procedures. We also thank R. Schwartz, Vacc
M et al.Author ManuscriptAuthor ManuscriptAuthor ManuscriptGautam et al.DFZWPage13.11.11.10.9.6.2.2.1.1.0.0.0.DFZV16.13.14.12.12.11.10.10.0.ACKNOWLEDGEMENTSWe thank R. Plishka, A. Peach and T. Lewis for determining plasma viral RNA loads and K. Rice, R. Engel, R. Petros and S. Fong for diligently assisting in the maintenance of animals and assisting with procedures. We also thank R. Schwartz, Vacc
The Robertson Foundation and HHMI.Nature. Author manuscript; available in PMC 2016 November 29.The plasma concentrations of the infused 10-1074 and VRC01-LS were measured longitudinally in the indicated animals.VRC01-LS conc (g/ml)DFXT14.12.12.11.10.9.9.7.6.5.5.4.3.2.1.33 0.10 23.0 0.9.8.8.7.6.4.4.4.2.1.1.1.Wks14.14.15.15.16.16.17.17.18.18.19.19.20.20.DFDP4.3.1.1.1.1.0.0.0.0.0.0.26.3 0.3.1.1.0.03P
E in PMC 2016 November 29.Extended Data TableVRC01 and 3BNC117 antibody concentrations in the plasma of macaques after a single administration of the indicated MAbs.3BNC117 conc (g/ml) DFVB 0.1 0.0 1.0 1.4 2.0 3.0 3.6 4.0 4.4 5.4 6.4 7.4 8.0 8.4 9.0 9.4 10.4 0.22 11.0 11.4 12.1 12.6 13.6 14.0 14.6 15.0 15.4 16.0 16.4 0.1 0.3 0.3 0.2 0.2 0.1 0.1 0.1 0.1 0.7 0.6 0.5 0.4 0.4 0.3 0.3 0.2 0.1 0.1 0.1 0