Use, F-31300, France. 3CHU de Toulouse, H ital Purpan, Laboratoire de Virologie, Toulouse, F-31300, France. 4CHU de Toulouse, H ital Purpan, Service des Maladies Infectieuses et Tropicales, Toulouse, F-31300, France. Correspondence and requests for materials should be addressed to S.R. (email: raymond.s@chu-toulouse.fr)Scientific RepoRts | 7:42215 | DOI: 10.1038/srepResultswww.nature.com/scientifi
Are located in a homopolymeric region. We evaluated the sensitivity of the NGS for quantifying CXCR4-using variants with artificial mixtures of pure X4 (CHS02) and R5 (CHS11) virus clones. The MiSeq NGS detected 0.5 of X4 viruses in 2/3 mixtures and 1 in 3/3 mixtures. The 454 GS-Junior NGS detected 1 of X4 viruses in 2/3 mixtures and 5 of X4 viruses in 3/3 mixtures (Table 1). Linear regression