Roth supports growth to an optical density at 600 nm (OD600) of 7, but exponential growth already slows down at an OD600 of 0.3. In the first short phase, the sugars are used for growth, while thereafter, the various catabolizable amino acids present in LB broth are consumed (549). The growth pattern in LB medium is complex, as a switch occurs from a sequential mode to a simultaneous mode of subst