Ir Immune Defic Syndr. Author manuscript; available in PMC 2013 June 01.Mosam et al.Pagena e, 89 had advanced (but not immediately life threatening) KS and 58 had CD4
To current convention [46]). C34-Ig was produced and purified as previously described [47]. The CD4-Ig fusion protein consists of the first two N-terminal domains of the CD4 molecule and the Fc region of human IgG1. Purification was carried out as described for the C34-Ig molecule [47].mechanism of sCD4 neutralization [17,18]. Resistance to sCD4 may thus arise by a decreased affinity of the envelo
He N-terminal residue of gp41) using the overlapping PCR method.Cell-Based Enzyme-Linked Immunosorbent Assay (ELISA)A sensitive cell-based ELISA with high temporal resolution was developed to measure the binding of antibodies to HIV-1 envelopeMetastable Activation of HIV-1 Envglycoprotein trimers expressed on cells. COS-1 cells were seeded in 96-well plates (2.46104 cells per well) and transfected