Nan (100 /ml) for 3 h at 37 in a 5 CO2 atmosphere. Cells were washed four times and autologous stimulated T cells were added onto HIV-exposed MDDC at a DC:T-cell ratio of 1:5. Each sample was performed in triplicate. Culture supernatants were harvested every 3 days and fresh medium was added. Supernatants were inactivated with 1 Triton X-100 and frozen at -20 . The viral production by T lympho
S) and cells were lysed (1 Triton X-100 for 45 min at 37 ). Cell lysates were harvested and centrifuged at 1,800 rpm for 5 min. The amount of HIV-p24 antigen associated to cell lysates was determined using the HIV-1 p24 core profile ELISA. HIV-1 attachment on MDDC to assess the attachment of HIV-1 to MDDC, the cells were washed 2 times after 6 days of differentiation and seeded into 96-well cultu
Incubation forsurvival = live cell number (test)/live cell number (control) ?Page 3 of(page number not for citation purposes)Journal of Translational Medicine 2007, 5:http://www.translational-medicine.com/content/5/1/min at 37 with 1 Triton X-100. Cell lysates were harvested and centrifuged at 1,800 rpm for 5 min. The amount of cell-associated HIV-1 was evaluated using p24 antigen capture ELISA.
Incubation forsurvival = live cell number (test)/live cell number (control) ?Page 3 of(page number not for citation purposes)Journal of Translational Medicine 2007, 5:http://www.translational-medicine.com/content/5/1/min at 37 with 1 Triton X-100. Cell lysates were harvested and centrifuged at 1,800 rpm for 5 min. The amount of cell-associated HIV-1 was evaluated using p24 antigen capture ELISA.
Lectins, participated in the design of the study, and helped draft the manuscript. LB and HS: conceived the study, participated in its design and coordination, and helped draft the manuscript. All authors read and approved the final manuscript.18. 15.16. 17.AcknowledgementsWe gratefully acknowledge Christophe Klein for technical assistance and Dr C ric Carbonneil for helpful discussions. This work
As hepatosplenomegaly. The maximum diameter of the paraaortic lymph nodes was 8 cm (Fig. 2A). We diagnosed a relapse of CLL based on the increased lymphocyte count and sIL-2R level in peripheral blood. As the patient's disease was refractory to the previous chemotherapies, bendamustine was administered at a dose of 70 mg/m2/day for 2 days and this was repeated on days 42?3, 98?9, and 182?83 [19] w
As hepatosplenomegaly. The maximum diameter of the paraaortic lymph nodes was 8 cm (Fig. 2A). We diagnosed a relapse of CLL based on the increased lymphocyte count and sIL-2R level in peripheral blood. As the patient's disease was refractory to the previous chemotherapies, bendamustine was administered at a dose of 70 mg/m2/day for 2 days and this was repeated on days 42?3, 98?9, and 182?83 [19] w