G Technology) at 1:1,000) in 2.5 milk. The next day, the membrane was rinsed with Tris-buffered saline (137 mM NaCl, 2.7 mM KCl, 19 mM Tric Base, pH 7.4) containing 0.1 Tween-20 and incubated with secondary anti-rabbit and anti-mouse antibodies (1:20,000 for both) conjugated with horseradish peroxidase. Immunoblots were visualized by chemiluminescence (GE Healthcare) and recorded on Fujifilm LAS
G Technology) at 1:1,000) in 2.5 milk. The next day, the membrane was rinsed with Tris-buffered saline (137 mM NaCl, 2.7 mM KCl, 19 mM Tric Base, pH 7.4) containing 0.1 Tween-20 and incubated with secondary anti-rabbit and anti-mouse antibodies (1:20,000 for both) conjugated with horseradish peroxidase. Immunoblots were visualized by chemiluminescence (GE Healthcare) and recorded on Fujifilm LAS
Including AACT, appear to promote tumor progression15. It was shown that tumors from a metastatic breastCancer. Author manuscript; available in PMC 2013 January 15.Roberts et al.Pagecancer cell line had higher levels of expression of AACT than those produced from a nonmetastatic cell line16. Additionally, malignant breast tumor cells induced local AACT expression by host cells at primary and secon
Including AACT, appear to promote tumor progression15. It was shown that tumors from a metastatic breastCancer. Author manuscript; available in PMC 2013 January 15.Roberts et al.Pagecancer cell line had higher levels of expression of AACT than those produced from a nonmetastatic cell line16. Additionally, malignant breast tumor cells induced local AACT expression by host cells at primary and secon