Nts in clinical practice but technical optimization are needed to improve quantification. Human immunodeficiency virus (HIV) enters its host cells following the interaction between the virus envelope glycoprotein (Gp120), the cell surface CD4 receptor, and a chemokine receptor, which may be CCR5 and/ or CXCR4, acting as a coreceptor1. The HIV tropism is defined by the coreceptor(s) use and is corr
Been fully validated for use in clinical practice. The Illumina NGS technology is used in many laboratories but its ability to predict HIV-1 tropism has not been evaluated while the 454 GS-Junior (Roche) is used for routine diagnosis. The genotypic prediction of HIV-1 tropism is based on sequencing the V3 region and interpreting the results with an appropriate algorithm. We compared the performanc
E: implications for the Pakistaniwww.ccsenet.org/gjhsGlobal Journal of Health ScienceVol. 5, No. 5;society. J. Pak. Med. Assoc., 61(3), 313-9. Hoodfar, H., Assadpour, S. (2000). The politics of population policy in the Islamic Republic of Iran. Stud. Fam. Plann., 31(1), 19-34. http://dx.doi.org/10.1111/j.1728-4465.2000.00019.x Huang, M., Hussein, H. (2004). The HIV/AIDS epidemic country paper:
E: implications for the Pakistaniwww.ccsenet.org/gjhsGlobal Journal of Health ScienceVol. 5, No. 5;society. J. Pak. Med. Assoc., 61(3), 313-9. Hoodfar, H., Assadpour, S. (2000). The politics of population policy in the Islamic Republic of Iran. Stud. Fam. Plann., 31(1), 19-34. http://dx.doi.org/10.1111/j.1728-4465.2000.00019.x Huang, M., Hussein, H. (2004). The HIV/AIDS epidemic country paper:
Recombinant virus phenotypic entry assay.Sensitivity of deep sequencing for detecting minor CXCR4-using variants. We determined the sensitivity threshold of NGS by calculating the error rate, either globally or for each position of V3. The mean frequency of V3 variant artifacts determined after analysis of 20 virus clones with the Illumina was 0.078 [exact Poisson 99 confidence interval (CI), 0.
Been fully validated for use in clinical practice. The Illumina NGS technology is used in many laboratories but its ability to predict HIV-1 tropism has not been evaluated while the 454 GS-Junior (Roche) is used for routine diagnosis. The genotypic prediction of HIV-1 tropism is based on sequencing the V3 region and interpreting the results with an appropriate algorithm. We compared the performanc
Use, F-31300, France. 3CHU de Toulouse, H ital Purpan, Laboratoire de Virologie, Toulouse, F-31300, France. 4CHU de Toulouse, H ital Purpan, Service des Maladies Infectieuses et Tropicales, Toulouse, F-31300, France. Correspondence and requests for materials should be addressed to S.R. (email: raymond.s@chu-toulouse.fr)Scientific RepoRts | 7:42215 | DOI: 10.1038/srepResultswww.nature.com/scientifi
Use, F-31300, France. 3CHU de Toulouse, H ital Purpan, Laboratoire de Virologie, Toulouse, F-31300, France. 4CHU de Toulouse, H ital Purpan, Service des Maladies Infectieuses et Tropicales, Toulouse, F-31300, France. Correspondence and requests for materials should be addressed to S.R. (email: raymond.s@chu-toulouse.fr)Scientific RepoRts | 7:42215 | DOI: 10.1038/srepResultswww.nature.com/scientifi