Us combinations of plasmids. Bar, 10 m. In the absence of a viral RNA, MS2-YFP had the appearance shown in Fig. 4A to C. (A) pFIVgag-CFP, pMS2-YFP, and pFIV -24. The nuclear envelope region (box, right panel) is shown enlarged below (scale bar, 5 m). (B) pFIVgag-CFP, pFP93, pMS2-YFP, and pFIV -24. (C and D) pFIVgag-CFP, pFP93, pMS2-YFP, and pFIV -24. Live-cell confocal imaging was performed 48 h a
Us combinations of plasmids. Bar, 10 m. In the absence of a viral RNA, MS2-YFP had the appearance shown in Fig. 4A to C. (A) pFIVgag-CFP, pMS2-YFP, and pFIV -24. The nuclear envelope region (box, right panel) is shown enlarged below (scale bar, 5 m). (B) pFIVgag-CFP, pFP93, pMS2-YFP, and pFIV -24. (C and D) pFIVgag-CFP, pFP93, pMS2-YFP, and pFIV -24. Live-cell confocal imaging was performed 48 h a
Tration of the study plant lectins in epithelial cell (A). MDM)line (HEC-1A) and primary immune cells (MDDC (A). Non-toxic concentration of the study plant lectins in epithelial cell line (HEC-1A) and primary immune cells (MDDC and MDM). HEC-1A and primary immune cells were cultured with concentrations of products for 24 h. After washing, culture viability was determined by using the MTT-cytotoxic
Er. Cells were incubated for 1 h at 37 . HIV-1 transcytosis was assessed by detecting the presence of p24 antigen (HIV-1 core profile ELISA) in the basolateral chamber of the transwell. The inhibition of transcytosis was expressed as the percentage of p24 antigen recovered in the basolateral chamber in the presence of each plant lectin by comparison to that recovered without the plant lectins. HIV
Er. Cells were incubated for 1 h at 37 . HIV-1 transcytosis was assessed by detecting the presence of p24 antigen (HIV-1 core profile ELISA) in the basolateral chamber of the transwell. The inhibition of transcytosis was expressed as the percentage of p24 antigen recovered in the basolateral chamber in the presence of each plant lectin by comparison to that recovered without the plant lectins. HIV
Bit Ability ofepithelial cells attachment of HIV-1 cell-free parAbility of lectins to inhibit attachment of HIV-1 cell-free particles on epithelial cells. HEC-1A cells were co-incubated with non-toxic concentrations of each lectins or mannan (100 /ml) and 5 ng of HIV-1Ba-L (A) or HIV-1NDK (B) virus were added for 1 h. Incubation with mannan was used as control. The quantity of attached virus was