Of plant lectins on HIV-1 transcytosis through a tight epithelial cells monolayer To evaluate the capacity of each plant lectin to inhibit in vitro HIV-1 transcytosis through genital epithelial cells, such as HEC-1A cells, we used a dual-chamber model in which the apical chamber consisted of a confluent monolayer of HEC-1A cells, and the basal chamber contained fresh medium. Cell-free virus (HIV-1
Of plant lectins on HIV-1 transcytosis through a tight epithelial cells monolayer To evaluate the capacity of each plant lectin to inhibit in vitro HIV-1 transcytosis through genital epithelial cells, such as HEC-1A cells, we used a dual-chamber model in which the apical chamber consisted of a confluent monolayer of HEC-1A cells, and the basal chamber contained fresh medium. Cell-free virus (HIV-1
The attachment phase which is (or are) (i) not an HIV-interacting GAG, as indicated by our observations in the presence of X4-tropic viruses, (ii) not C-type lectin(s), considering the absenceDiscussionThe plan lectins HHA and GNA constitute two promising microbicide molecules of great interest, capable to efficiently inhibit the infection of T lymphocytes and PBMC by a broad range of HIV strains
The attachment phase which is (or are) (i) not an HIV-interacting GAG, as indicated by our observations in the presence of X4-tropic viruses, (ii) not C-type lectin(s), considering the absenceDiscussionThe plan lectins HHA and GNA constitute two promising microbicide molecules of great interest, capable to efficiently inhibit the infection of T lymphocytes and PBMC by a broad range of HIV strains
L side of the transwell, and epithelial integrity was measured at 30 min and 2, 4, 9, and 24 h by using the Minicell-ERS instrument. The data shown are the averages from duplicate wells ?1 standard error of the mean after product addition.Page 4 of(page number not for citation purposes)Journal of Translational Medicine 2007, 5:http://www.translational-medicine.com/content/5/1/p24 (pg/ml)of the pla
Bit Ability ofepithelial cells attachment of HIV-1 cell-free parAbility of lectins to inhibit attachment of HIV-1 cell-free particles on epithelial cells. HEC-1A cells were co-incubated with non-toxic concentrations of each lectins or mannan (100 /ml) and 5 ng of HIV-1Ba-L (A) or HIV-1NDK (B) virus were added for 1 h. Incubation with mannan was used as control. The quantity of attached virus was