Ated for animals receiving no antibodies. The plasma neutralization titer was also determined for each of the MAb recipients at multiple times post infusion (Figure 4a). The median plasma neutralizing titers for the four groups of macaques at the time of SHIVAD8-EO acquisition were low:
Me transcription through the provirus, since slight enrichment of elongating RNAPII in the HIV coding region of transcriptionally silent J-Lat 9.2 cells was observed using ChIPqPCR assay. However, transcription originating from the host promoter, ignoring pA sites in both LTRs and consequently splicing out the provirus together with the host intron (Han et al., 2004) is most likely less frequent t
Ranscription units (Han et al., 2004; Lewinski et al., 2005). In the present work, we extended this knowledge by a detailed analysis of how TI impacts viral transcription in latent cells. Although TI occurs regardless of the orientation of the two promoters, we studied TI phenomenon in two J-Lat cell lines with the same orientation of the host and viral promoters, which allowed us to detect and an
Me transcription through the provirus, since slight enrichment of elongating RNAPII in the HIV coding region of transcriptionally silent J-Lat 9.2 cells was observed using ChIPqPCR assay. However, transcription originating from the host promoter, ignoring pA sites in both LTRs and consequently splicing out the provirus together with the host intron (Han et al., 2004) is most likely less frequent t
E in PMC 2016 November 29.Extended Data TableVRC01 and 3BNC117 antibody concentrations in the plasma of macaques after a single administration of the indicated MAbs.3BNC117 conc (g/ml) DFVB 0.1 0.0 1.0 1.4 2.0 3.0 3.6 4.0 4.4 5.4 6.4 7.4 8.0 8.4 9.0 9.4 10.4 0.22 11.0 11.4 12.1 12.6 13.6 14.0 14.6 15.0 15.4 16.0 16.4 0.1 0.3 0.3 0.2 0.2 0.1 0.1 0.1 0.1 0.7 0.6 0.5 0.4 0.4 0.3 0.3 0.2 0.1 0.1 0.1 0
E in PMC 2016 November 29.Extended Data TableVRC01 and 3BNC117 antibody concentrations in the plasma of macaques after a single administration of the indicated MAbs.3BNC117 conc (g/ml) DFVB 0.1 0.0 1.0 1.4 2.0 3.0 3.6 4.0 4.4 5.4 6.4 7.4 8.0 8.4 9.0 9.4 10.4 0.22 11.0 11.4 12.1 12.6 13.6 14.0 14.6 15.0 15.4 16.0 16.4 0.1 0.3 0.3 0.2 0.2 0.1 0.1 0.1 0.1 0.7 0.6 0.5 0.4 0.4 0.3 0.3 0.2 0.1 0.1 0.1 0
Ormed using NorthernMax kit (Ambion) according to the manufacturer's instructions. The probes were amplified from cDNA synthesized with MMLV reverse transcriptase (Invitrogen). PCR products were labeled with BioNick Labelling System (Invitrogen). Stimulation of J-Lat cells and detection of activated cells by flow cytometry Tat protein was expressed from pCDNA3 vector using electroporation of 107 J
Ormed using NorthernMax kit (Ambion) according to the manufacturer's instructions. The probes were amplified from cDNA synthesized with MMLV reverse transcriptase (Invitrogen). PCR products were labeled with BioNick Labelling System (Invitrogen). Stimulation of J-Lat cells and detection of activated cells by flow cytometry Tat protein was expressed from pCDNA3 vector using electroporation of 107 J