Ns dormant. In contrast, we demonstrated that TI could be counteracted by specific inhibition of the upstream transcription or by cooperative activation of transcription initiation and elongation from the 5'LTR by the viral and host TFs. In the latter scenario, we envision that RNAPII initiating from the viral promoter competes successfully with the RNAPII that is elongating through the 5'LTR. Bec
Ed cells. Furthermore, TNF- stimulation led to the appearance of Sp1 and initiating RNAPII on the 5'LTR and additional enrichments of initiating and elongating RNAPII on the 3'LTR. These effects are most likely achieved through the activation of NF-B, which stimulates several steps of viral transcription including the PIC formation, transcription initiation and transcription elongation. Thus, our
Tion at the time of each challenge using a probit regression model. The fitted probabilities from the models are plotted separately for each MAb group, with the estimated probability of infection for the control animals (0.27) indicated by the open circle adjacent to each ordinate. The VRC01 and VRC01-LS curves are superimposed. The points on each curve represent the median concentration at the ti
Ranscription units (Han et al., 2004; Lewinski et al., 2005). In the present work, we extended this knowledge by a detailed analysis of how TI impacts viral transcription in latent cells. Although TI occurs regardless of the orientation of the two promoters, we studied TI phenomenon in two J-Lat cell lines with the same orientation of the host and viral promoters, which allowed us to detect and an
Ormed using NorthernMax kit (Ambion) according to the manufacturer's instructions. The probes were amplified from cDNA synthesized with MMLV reverse transcriptase (Invitrogen). PCR products were labeled with BioNick Labelling System (Invitrogen). Stimulation of J-Lat cells and detection of activated cells by flow cytometry Tat protein was expressed from pCDNA3 vector using electroporation of 107 J
Ormed using NorthernMax kit (Ambion) according to the manufacturer's instructions. The probes were amplified from cDNA synthesized with MMLV reverse transcriptase (Invitrogen). PCR products were labeled with BioNick Labelling System (Invitrogen). Stimulation of J-Lat cells and detection of activated cells by flow cytometry Tat protein was expressed from pCDNA3 vector using electroporation of 107 J
E in PMC 2016 November 29.Extended Data TableVRC01 and 3BNC117 antibody concentrations in the plasma of macaques after a single administration of the indicated MAbs.3BNC117 conc (g/ml) DFVB 0.1 0.0 1.0 1.4 2.0 3.0 3.6 4.0 4.4 5.4 6.4 7.4 8.0 8.4 9.0 9.4 10.4 0.22 11.0 11.4 12.1 12.6 13.6 14.0 14.6 15.0 15.4 16.0 16.4 0.1 0.3 0.3 0.2 0.2 0.1 0.1 0.1 0.1 0.7 0.6 0.5 0.4 0.4 0.3 0.3 0.2 0.1 0.1 0.1 0
E in PMC 2016 November 29.Extended Data TableVRC01 and 3BNC117 antibody concentrations in the plasma of macaques after a single administration of the indicated MAbs.3BNC117 conc (g/ml) DFVB 0.1 0.0 1.0 1.4 2.0 3.0 3.6 4.0 4.4 5.4 6.4 7.4 8.0 8.4 9.0 9.4 10.4 0.22 11.0 11.4 12.1 12.6 13.6 14.0 14.6 15.0 15.4 16.0 16.4 0.1 0.3 0.3 0.2 0.2 0.1 0.1 0.1 0.1 0.7 0.6 0.5 0.4 0.4 0.3 0.3 0.2 0.1 0.1 0.1 0