Tion can then be used to serve the covalent attachment of protein to thesupport. The physical means of freeing the protein from the streptavidin complex is unique to those proteins having a terminal alkyne because only those adducts bearing the alkyne are linked to the photolabile group by click chemistry. The photochemical reaction applied is the benzoin photocleavage first studied by Sheehan (26
Led into full-length sequences using a polymerase chain reaction (PCR)-like process (Stemmer, 1994). In directed evolution, the cap genes from multiple AAV serotypes are fragmented, reannealed using a primerless PCR reaction, and then in a second PCR reaction are assembled into full-length chimeric cap genes. Chimeric viral libraries produced in this manner can then be administered systemically, t
Erence in visualizing punctate versus diffuse structures, the intensity settings were not the same for cells treated with DMSO versus 1 M GCA. Using the same settings, GM130 could not be visualized in the 1 M GCA-treated cells without saturating the GM130 in the DMSO-treated cells. Results shown are representative of two experiments in which cells were incubated in the presence of drugs for 16 h a
In Gc pathogenesis remain to be examined. The gonococcal -2,3-sialyltransferase Lst transfers sialyl groups from host-derived CMP-N-acetylneuraminic acid to the terminal galactose residue on the oligosaccharide of LOS (Gilbert et al., 1996). Sialylation contributes to Gc resistance to normal human serum as well as PMN-derived oxygen-independent antimicrobial factors (Shafer et al., 1986; Parsons e