L V3 variants of 20 virus clones. The position mean (red line) is the error rate estimated at each position of V3 by comparing the UDS reads to the Sanger sequences of 20 clones. The shaded regions represent the 99 confidence interval of global (blue) and position (red) mean error rates.compare the systems. The mean frequency of V3 variant artifacts found with the 454 GS-Junior was 0.018 [exact
Ic? Findings from a population based study in Karachi, Pakistan. BMC Infect. Dis., 9, 38. http://dx.doi.org/10.1186/1471-2334-9-38 Fox, M. P., Mazimba, A., Seidenberg, P., Crooks, D., Sikateyo, B., Rosen, S. (2010). Barriers to initiation of antiretroviral treatment in rural and urban areas of Zambia: a cross-sectional study of cost, stigma, and perceptions about ART. J. Int. AIDS Soc., 13, 8. h
Ic? Findings from a population based study in Karachi, Pakistan. BMC Infect. Dis., 9, 38. http://dx.doi.org/10.1186/1471-2334-9-38 Fox, M. P., Mazimba, A., Seidenberg, P., Crooks, D., Sikateyo, B., Rosen, S. (2010). Barriers to initiation of antiretroviral treatment in rural and urban areas of Zambia: a cross-sectional study of cost, stigma, and perceptions about ART. J. Int. AIDS Soc., 13, 8. h
Nding gendered influences on women's reproductive health in Pakistan: moving beyond the autonomy paradigm. Soc. Sci. Med., 68(7), 1349-56. http://dx.doi.org/10.1016/j.socscimed.2009.01.025 National Institute of Population Studies (NIPS) Islamabad Pakistan Macro International Inc. USA (June 2008). Pakistan Demographic and Health Survey 2006-07. Patton, M. Q. (1999). Enhancing the quality and credib
Coreceptor use. The sequence reads were aligned with the BaL consen-Scientific RepoRts | 7:42215 | DOI: 10.1038/srepwww.nature.com/scientificreports/number of negatively charged amino acids (D and E) from the number of positively charged ones (K and R). The CM classifier (generic prediction) was used to predict tropism with cleaned data of V3 amino acid sequences20.Determining the position-specifi
Coreceptor use. The sequence reads were aligned with the BaL consen-Scientific RepoRts | 7:42215 | DOI: 10.1038/srepwww.nature.com/scientificreports/number of negatively charged amino acids (D and E) from the number of positively charged ones (K and R). The CM classifier (generic prediction) was used to predict tropism with cleaned data of V3 amino acid sequences20.Determining the position-specifi
Ion sequencing platforms for determining HIV-1 coreceptor useSt hanie Raymond1,2,3, Florence Nicot3, Nicolas Jeanne3, Olivier Delfour3, Romain Carcenac3, Caroline Lefebvre3, Michelle Cazabat1,3, Karine Saun?,2,3, Pierre Delobel1,2,4 Jacques Izopet1,2,The coreceptor used by HIV-1 must be determined before a CCR5 antagonist, part of the arsenal of antiretroviral drugs, is prescribed because viruse
Nts in clinical practice but technical optimization are needed to improve quantification. Human immunodeficiency virus (HIV) enters its host cells following the interaction between the virus envelope glycoprotein (Gp120), the cell surface CD4 receptor, and a chemokine receptor, which may be CCR5 and/ or CXCR4, acting as a coreceptor1. The HIV tropism is defined by the coreceptor(s) use and is corr