O (with the extent of decrease dependent on the relative efficiency of cleavage by "basal" versus "experimental" proteases) and thus lower the chance of cleavage fragment detection. This scenario is illustrated by examples of thrombospondin-2 and IGFBP-4 where several low ratio peptides were confirmed by Edman sequencing to be the products of true cleavage events (Figs. 4 and 6). The second limiti
O (with the extent of decrease dependent on the relative efficiency of cleavage by "basal" versus "experimental" proteases) and thus lower the chance of cleavage fragment detection. This scenario is illustrated by examples of thrombospondin-2 and IGFBP-4 where several low ratio peptides were confirmed by Edman sequencing to be the products of true cleavage events (Figs. 4 and 6). The second limiti
Ve CRE sites were able to bind recombinant CRE binding-bZIP proteins, conditions under which these CRE sites come into play in native neurons are unknown. GluA1 receptors (like all AMPA receptor subunits) are expressed by neurons and glial cells in vivo and in vitro (Gallo and Ghiani, 2000), but the density of functional receptors is much lower in astrocytes than neurons. In oligodendrocyte progen
Es to pass this cutoff value in each of three biological replicates. Analytical reproducibility was addressed by running the Glu-1-Fibrinopeptide B standard peptide (Glu-Fib, amino acid sequence: EGVNDNEEGFFSAR, monoisotopic m/z 1770.68) at least between every six runs, and usually between every two to three runs. The Glu-Fib 2 ion (m/z 785.8421) was monitored and verified to be within 3 ppm error
Translocator proteins in P. aeruginosa or S. typhimuriurm, does not result in loss of effector secretion control (Broms et al., 2003, Cisz et al., 2008, Kaniga et al., 1995b). Another variation of the plug model has been proposed, where a `sensor' protein has been inserted into the type III secretion channel (Blocker et al., 2008). Its export is inhibited by the type III secretion needle-tip and i
Translocator proteins in P. aeruginosa or S. typhimuriurm, does not result in loss of effector secretion control (Broms et al., 2003, Cisz et al., 2008, Kaniga et al., 1995b). Another variation of the plug model has been proposed, where a `sensor' protein has been inserted into the type III secretion channel (Blocker et al., 2008). Its export is inhibited by the type III secretion needle-tip and i
Across mucosal-epithelial borders as in the oro-naso-pharynx, gut, lung, or kidney; physical breaks in barriers such as the skin; ascending infection through the urogenital tract of females resulting in dissemination from the fallopian tubes; and through direct access to the bloodstream via indwelling catheters or devices. Clinically, BSI is often categorized in 2 primary ways: 1) by origin of the
Cell cycle proteins. Proteins were mapped to the cell cycle pathway according to the KEGG database together with their abundance classes determined from the analysis of unsynchronized proliferating cells (Fig. 1A, Experiment 2). Proteins not identified in the PeptideAtlas are indicated in white.FIG. 4. Correlation of protein and mRNA levels. Scatterplot of normalized mRNA abundances and normalized