Thus, the median times to virus acquisition compared to control monkeys were 8 weeks for VRC01, 13 weeks for 3BNC117 and 12.5 weeks for 10-1074. The pharmacokinetic profile of VRC01 was altered by introducing two amino acid mutations (M428L and N434S, referred to as "LS"), into its Fc domain, which increased its half-life in both plasma and tissues9. The neutralization activity of this VRC01 deriv
Thus, the median times to virus acquisition compared to control monkeys were 8 weeks for VRC01, 13 weeks for 3BNC117 and 12.5 weeks for 10-1074. The pharmacokinetic profile of VRC01 was altered by introducing two amino acid mutations (M428L and N434S, referred to as "LS"), into its Fc domain, which increased its half-life in both plasma and tissues9. The neutralization activity of this VRC01 deriv
Protection has, in fact, been achieved by administering polyclonal and monoclonal antiHIV-1 neutralizing antibodies (NAbs) to humanized mice or macaques prior to challenge with SIV/HIV chimeric viruses (SHIVs)1-8. During the past seven years, monoclonal antibodies (MAbs) have been isolated from selected HIV-1 infected individuals, who generate anti-viral NAbs (bNAbs) with broad and potent activity
Protection has, in fact, been achieved by administering polyclonal and monoclonal antiHIV-1 neutralizing antibodies (NAbs) to humanized mice or macaques prior to challenge with SIV/HIV chimeric viruses (SHIVs)1-8. During the past seven years, monoclonal antibodies (MAbs) have been isolated from selected HIV-1 infected individuals, who generate anti-viral NAbs (bNAbs) with broad and potent activity
Lation than the 5'LTR, suggesting that the latter LTR is much stronger subjected to TI. Even if there is transcription from host gene `reading through' both LTRs in some latent cells, this does not diminish the impact of TI on the 5'LTR.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptCell Host Microbe. Author manuscript; available in PMC 2014 November 03.Lenasi et al.Page
Lation than the 5'LTR, suggesting that the latter LTR is much stronger subjected to TI. Even if there is transcription from host gene `reading through' both LTRs in some latent cells, this does not diminish the impact of TI on the 5'LTR.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptCell Host Microbe. Author manuscript; available in PMC 2014 November 03.Lenasi et al.Page
Ns dormant. In contrast, we demonstrated that TI could be counteracted by specific inhibition of the upstream transcription or by cooperative activation of transcription initiation and elongation from the 5'LTR by the viral and host TFs. In the latter scenario, we envision that RNAPII initiating from the viral promoter competes successfully with the RNAPII that is elongating through the 5'LTR. Bec
Ns dormant. In contrast, we demonstrated that TI could be counteracted by specific inhibition of the upstream transcription or by cooperative activation of transcription initiation and elongation from the 5'LTR by the viral and host TFs. In the latter scenario, we envision that RNAPII initiating from the viral promoter competes successfully with the RNAPII that is elongating through the 5'LTR. Bec