Composition of the protein medium (plasma, serum), its concentration (1 , 10 ) and whether filtered or not, affected the profiles of proteins eluted from surfaces having different SAM chemistries. We have previously demonstrated that the lowest DC maturation and highest T cell tolerization was observed on CH3 SAM surfaces43. The protein profiles in CH3 SAM eluates observed in this study support th
Irment of vision. The mechanisms of DR-related vision loss include vitreous hemorrhage, tractional retinal detachment from proliferative diabetic retinopathy, development of a fibrovascular membrane in the vitreous, and macular edema. Study of diabetic neovascularization and macular edema in laboratory animals has been problematic, as most laboratory species lack a macula, and have not shown the r
Irment of vision. The mechanisms of DR-related vision loss include vitreous hemorrhage, tractional retinal detachment from proliferative diabetic retinopathy, development of a fibrovascular membrane in the vitreous, and macular edema. Study of diabetic neovascularization and macular edema in laboratory animals has been problematic, as most laboratory species lack a macula, and have not shown the r
Proteomes exhibit a 2 net charge at pH 2.7 and can be separated from other peptides according to single charge differences using SCX chromatography (33). Because trypsin cuts poorly at Meth-R, the missed cleavages produce peptides with charge 3 at pH 2.7. Thus, most Meth-R peptides should be present in the 3 peptide-containing fractions after SCX chromatography. SCX fractions enriched for tryptic
Ies). SEQUEST and X!Tandem were searched with a fragment ion mass tolerance of 0.80 Da and a parent ion tolerance of 10.0PPM. Iodoacetamide derivative of cysteine was specified as a fixed modification, whereas oxidation of methionine was specified as a variable modification. Results were merged using Scaffold (Proteome Software) version 3.0.8 which calculated False Discovery Rates (FDRs) using a r