As hepatosplenomegaly. The maximum diameter of the paraaortic lymph nodes was 8 cm (Fig. 2A). We diagnosed a relapse of CLL based on the increased lymphocyte count and sIL-2R level in peripheral blood. As the patient's disease was refractory to the previous chemotherapies, bendamustine was administered at a dose of 70 mg/m2/day for 2 days and this was repeated on days 42?3, 98?9, and 182?83 [19] w
As hepatosplenomegaly. The maximum diameter of the paraaortic lymph nodes was 8 cm (Fig. 2A). We diagnosed a relapse of CLL based on the increased lymphocyte count and sIL-2R level in peripheral blood. As the patient's disease was refractory to the previous chemotherapies, bendamustine was administered at a dose of 70 mg/m2/day for 2 days and this was repeated on days 42?3, 98?9, and 182?83 [19] w
Lectins, participated in the design of the study, and helped draft the manuscript. LB and HS: conceived the study, participated in its design and coordination, and helped draft the manuscript. All authors read and approved the final manuscript.18. 15.16. 17.AcknowledgementsWe gratefully acknowledge Christophe Klein for technical assistance and Dr C ric Carbonneil for helpful discussions. This work
Ch lectin to interact with epithelial cell's receptors, labeled HHA and GNA were incubated with epithelial cells. As depicted in Figure 2C, only HHA but not GNA interacted with receptors expressed at the surface of HEC-1. Moreover, the preincubation of GNA with epithelial cells followed with several washes did not inhibit viral attachment whereas the preincubation of GNA with R5 viral particles in
O HIV-1. The 24 stem-loops were similarly inserted in an HIV-1 vector, and a packaging-deficient version was created by deleting the four adjacent stemloops (SL1 to SL4) upstream of the 5 portion of the gag gene and 40 bp of the gag gene (Fig. 1E). To visualize HIV-1 Gag, we fused CFP to its C terminus (Fig. 1F). Similar to FIV Gag-CFP, HIV-1 Gag-CFP is incorporated into viral particles (data not
O HIV-1. The 24 stem-loops were similarly inserted in an HIV-1 vector, and a packaging-deficient version was created by deleting the four adjacent stemloops (SL1 to SL4) upstream of the 5 portion of the gag gene and 40 bp of the gag gene (Fig. 1E). To visualize HIV-1 Gag, we fused CFP to its C terminus (Fig. 1F). Similar to FIV Gag-CFP, HIV-1 Gag-CFP is incorporated into viral particles (data not
Us combinations of plasmids. Bar, 10 m. In the absence of a viral RNA, MS2-YFP had the appearance shown in Fig. 4A to C. (A) pFIVgag-CFP, pMS2-YFP, and pFIV -24. The nuclear envelope region (box, right panel) is shown enlarged below (scale bar, 5 m). (B) pFIVgag-CFP, pFP93, pMS2-YFP, and pFIV -24. (C and D) pFIVgag-CFP, pFP93, pMS2-YFP, and pFIV -24. Live-cell confocal imaging was performed 48 h a
Us combinations of plasmids. Bar, 10 m. In the absence of a viral RNA, MS2-YFP had the appearance shown in Fig. 4A to C. (A) pFIVgag-CFP, pMS2-YFP, and pFIV -24. The nuclear envelope region (box, right panel) is shown enlarged below (scale bar, 5 m). (B) pFIVgag-CFP, pFP93, pMS2-YFP, and pFIV -24. (C and D) pFIVgag-CFP, pFP93, pMS2-YFP, and pFIV -24. Live-cell confocal imaging was performed 48 h a