Incubation forsurvival = live cell number (test)/live cell number (control) ?Page 3 of(page number not for citation purposes)Journal of Translational Medicine 2007, 5:http://www.translational-medicine.com/content/5/1/min at 37 with 1 Triton X-100. Cell lysates were harvested and centrifuged at 1,800 rpm for 5 min. The amount of cell-associated HIV-1 was evaluated using p24 antigen capture ELISA.
Incubation forsurvival = live cell number (test)/live cell number (control) ?Page 3 of(page number not for citation purposes)Journal of Translational Medicine 2007, 5:http://www.translational-medicine.com/content/5/1/min at 37 with 1 Triton X-100. Cell lysates were harvested and centrifuged at 1,800 rpm for 5 min. The amount of cell-associated HIV-1 was evaluated using p24 antigen capture ELISA.
Ch lectin to interact with epithelial cell's receptors, labeled HHA and GNA were incubated with epithelial cells. As depicted in Figure 2C, only HHA but not GNA interacted with receptors expressed at the surface of HEC-1. Moreover, the preincubation of GNA with epithelial cells followed with several washes did not inhibit viral attachment whereas the preincubation of GNA with R5 viral particles in
O HIV-1. The 24 stem-loops were similarly inserted in an HIV-1 vector, and a packaging-deficient version was created by deleting the four adjacent stemloops (SL1 to SL4) upstream of the 5 portion of the gag gene and 40 bp of the gag gene (Fig. 1E). To visualize HIV-1 Gag, we fused CFP to its C terminus (Fig. 1F). Similar to FIV Gag-CFP, HIV-1 Gag-CFP is incorporated into viral particles (data not
O HIV-1. The 24 stem-loops were similarly inserted in an HIV-1 vector, and a packaging-deficient version was created by deleting the four adjacent stemloops (SL1 to SL4) upstream of the 5 portion of the gag gene and 40 bp of the gag gene (Fig. 1E). To visualize HIV-1 Gag, we fused CFP to its C terminus (Fig. 1F). Similar to FIV Gag-CFP, HIV-1 Gag-CFP is incorporated into viral particles (data not
Us combinations of plasmids. Bar, 10 m. In the absence of a viral RNA, MS2-YFP had the appearance shown in Fig. 4A to C. (A) pFIVgag-CFP, pMS2-YFP, and pFIV -24. The nuclear envelope region (box, right panel) is shown enlarged below (scale bar, 5 m). (B) pFIVgag-CFP, pFP93, pMS2-YFP, and pFIV -24. (C and D) pFIVgag-CFP, pFP93, pMS2-YFP, and pFIV -24. Live-cell confocal imaging was performed 48 h a
Us combinations of plasmids. Bar, 10 m. In the absence of a viral RNA, MS2-YFP had the appearance shown in Fig. 4A to C. (A) pFIVgag-CFP, pMS2-YFP, and pFIV -24. The nuclear envelope region (box, right panel) is shown enlarged below (scale bar, 5 m). (B) pFIVgag-CFP, pFP93, pMS2-YFP, and pFIV -24. (C and D) pFIVgag-CFP, pFP93, pMS2-YFP, and pFIV -24. Live-cell confocal imaging was performed 48 h a
Plant lectins. The HIV-1J-RCSF strain was used in this experiment due to the low transcytosis ability of HIV-1Ba-L strain [8]. As shown in Figure 3A, HHA inhibited transcytosis of cell-free HIV-1 in a dose-dependent manner. Similarly to mannan (100 /ml) that inhibited HIV transcytosis up to 41 , increasing concentrations of HHA (range 1-10-100 /ml) afforded a 17 , 42 and 54 decrease of HIV-1JR