Incubation forsurvival = live cell number (test)/live cell number (control) ?Page 3 of(page number not for citation purposes)Journal of Translational Medicine 2007, 5:http://www.translational-medicine.com/content/5/1/min at 37 with 1 Triton X-100. Cell lysates were harvested and centrifuged at 1,800 rpm for 5 min. The amount of cell-associated HIV-1 was evaluated using p24 antigen capture ELISA.
Incubation forsurvival = live cell number (test)/live cell number (control) ?Page 3 of(page number not for citation purposes)Journal of Translational Medicine 2007, 5:http://www.translational-medicine.com/content/5/1/min at 37 with 1 Triton X-100. Cell lysates were harvested and centrifuged at 1,800 rpm for 5 min. The amount of cell-associated HIV-1 was evaluated using p24 antigen capture ELISA.
Incubation forsurvival = live cell number (test)/live cell number (control) ?Page 3 of(page number not for citation purposes)Journal of Translational Medicine 2007, 5:http://www.translational-medicine.com/content/5/1/min at 37 with 1 Triton X-100. Cell lysates were harvested and centrifuged at 1,800 rpm for 5 min. The amount of cell-associated HIV-1 was evaluated using p24 antigen capture ELISA.
Incubation forsurvival = live cell number (test)/live cell number (control) ?Page 3 of(page number not for citation purposes)Journal of Translational Medicine 2007, 5:http://www.translational-medicine.com/content/5/1/min at 37 with 1 Triton X-100. Cell lysates were harvested and centrifuged at 1,800 rpm for 5 min. The amount of cell-associated HIV-1 was evaluated using p24 antigen capture ELISA.
S) and cells were lysed (1 Triton X-100 for 45 min at 37 ). Cell lysates were harvested and centrifuged at 1,800 rpm for 5 min. The amount of HIV-p24 antigen associated to cell lysates was determined using the HIV-1 p24 core profile ELISA. HIV-1 attachment on MDDC to assess the attachment of HIV-1 to MDDC, the cells were washed 2 times after 6 days of differentiation and seeded into 96-well cultu
Nan (100 /ml) for 3 h at 37 in a 5 CO2 atmosphere. Cells were washed four times and autologous stimulated T cells were added onto HIV-exposed MDDC at a DC:T-cell ratio of 1:5. Each sample was performed in triplicate. Culture supernatants were harvested every 3 days and fresh medium was added. Supernatants were inactivated with 1 Triton X-100 and frozen at -20 . The viral production by T lympho
Plant lectins. The HIV-1J-RCSF strain was used in this experiment due to the low transcytosis ability of HIV-1Ba-L strain [8]. As shown in Figure 3A, HHA inhibited transcytosis of cell-free HIV-1 in a dose-dependent manner. Similarly to mannan (100 /ml) that inhibited HIV transcytosis up to 41 , increasing concentrations of HHA (range 1-10-100 /ml) afforded a 17 , 42 and 54 decrease of HIV-1JR
Us combinations of plasmids. Bar, 10 m. In the absence of a viral RNA, MS2-YFP had the appearance shown in Fig. 4A to C. (A) pFIVgag-CFP, pMS2-YFP, and pFIV -24. The nuclear envelope region (box, right panel) is shown enlarged below (scale bar, 5 m). (B) pFIVgag-CFP, pFP93, pMS2-YFP, and pFIV -24. (C and D) pFIVgag-CFP, pFP93, pMS2-YFP, and pFIV -24. Live-cell confocal imaging was performed 48 h a