As hepatosplenomegaly. The maximum diameter of the paraaortic lymph nodes was 8 cm (Fig. 2A). We diagnosed a relapse of CLL based on the increased lymphocyte count and sIL-2R level in peripheral blood. As the patient's disease was refractory to the previous chemotherapies, bendamustine was administered at a dose of 70 mg/m2/day for 2 days and this was repeated on days 42?3, 98?9, and 182?83 [19] w
L side of the transwell, and epithelial integrity was measured at 30 min and 2, 4, 9, and 24 h by using the Minicell-ERS instrument. The data shown are the averages from duplicate wells ?1 standard error of the mean after product addition.Page 4 of(page number not for citation purposes)Journal of Translational Medicine 2007, 5:http://www.translational-medicine.com/content/5/1/p24 (pg/ml)of the pla
Achment on HEC-1A Considering that both HHA and GNA were non-toxic in the epithelial cell system, we further investigated the inhibition of HIV adsorption on the apical side of HEC-1A by the compounds. Cells were thus incubated with HIV-1Ba-L in the presence or absence of different concentrations of HHA or GNA. As depicted in Figure 2A, HHA faintly decreased the attachment of HIV-1Ba-L on epitheli
Achment on HEC-1A Considering that both HHA and GNA were non-toxic in the epithelial cell system, we further investigated the inhibition of HIV adsorption on the apical side of HEC-1A by the compounds. Cells were thus incubated with HIV-1Ba-L in the presence or absence of different concentrations of HHA or GNA. As depicted in Figure 2A, HHA faintly decreased the attachment of HIV-1Ba-L on epitheli
Ation MDDC are washed and 2 ?105 cells were adsorbed on a microscopy-adapted slide. Cells were incubated with or without HHA-TRITC or GNA-TRITC (200 /ml; Eylabs) at 4 for 30 min. Cells were washed with PBS 0.01 azide 0.5 BSA and then fixed with 1 paraformaldehyde. The coverslides were mounted in Mowiol (SigmaAldrich). Fluorescence analysis was performed with a Zeiss LSM510 confocal microscope
Compared with those of standard OD490 versus cell number curves generated for each cell type. The percentage survival was calculated using the formula :Epithelial monolayer integrity the effect of the plant lectins on their ability to maintain an intact epithelium was determined by measuring transepithelial resistance (TER). HEC-1A cells were grown as a tight polarized monolayer on a permeable sup
Compared with those of standard OD490 versus cell number curves generated for each cell type. The percentage survival was calculated using the formula :Epithelial monolayer integrity the effect of the plant lectins on their ability to maintain an intact epithelium was determined by measuring transepithelial resistance (TER). HEC-1A cells were grown as a tight polarized monolayer on a permeable sup
Compared with those of standard OD490 versus cell number curves generated for each cell type. The percentage survival was calculated using the formula :Epithelial monolayer integrity the effect of the plant lectins on their ability to maintain an intact epithelium was determined by measuring transepithelial resistance (TER). HEC-1A cells were grown as a tight polarized monolayer on a permeable sup