Are located in a homopolymeric region. We evaluated the sensitivity of the NGS for quantifying CXCR4-using variants with artificial mixtures of pure X4 (CHS02) and R5 (CHS11) virus clones. The MiSeq NGS detected 0.5 of X4 viruses in 2/3 mixtures and 1 in 3/3 mixtures. The 454 GS-Junior NGS detected 1 of X4 viruses in 2/3 mixtures and 5 of X4 viruses in 3/3 mixtures (Table 1). Linear regression
Are located in a homopolymeric region. We evaluated the sensitivity of the NGS for quantifying CXCR4-using variants with artificial mixtures of pure X4 (CHS02) and R5 (CHS11) virus clones. The MiSeq NGS detected 0.5 of X4 viruses in 2/3 mixtures and 1 in 3/3 mixtures. The 454 GS-Junior NGS detected 1 of X4 viruses in 2/3 mixtures and 5 of X4 viruses in 3/3 mixtures (Table 1). Linear regression
L V3 variants of 20 virus clones. The position mean (red line) is the error rate estimated at each position of V3 by comparing the UDS reads to the Sanger sequences of 20 clones. The shaded regions represent the 99 confidence interval of global (blue) and position (red) mean error rates.compare the systems. The mean frequency of V3 variant artifacts found with the 454 GS-Junior was 0.018 [exact
E: implications for the Pakistaniwww.ccsenet.org/gjhsGlobal Journal of Health ScienceVol. 5, No. 5;society. J. Pak. Med. Assoc., 61(3), 313-9. Hoodfar, H., Assadpour, S. (2000). The politics of population policy in the Islamic Republic of Iran. Stud. Fam. Plann., 31(1), 19-34. http://dx.doi.org/10.1111/j.1728-4465.2000.00019.x Huang, M., Hussein, H. (2004). The HIV/AIDS epidemic country paper:
E: implications for the Pakistaniwww.ccsenet.org/gjhsGlobal Journal of Health ScienceVol. 5, No. 5;society. J. Pak. Med. Assoc., 61(3), 313-9. Hoodfar, H., Assadpour, S. (2000). The politics of population policy in the Islamic Republic of Iran. Stud. Fam. Plann., 31(1), 19-34. http://dx.doi.org/10.1111/j.1728-4465.2000.00019.x Huang, M., Hussein, H. (2004). The HIV/AIDS epidemic country paper:
E: implications for the Pakistaniwww.ccsenet.org/gjhsGlobal Journal of Health ScienceVol. 5, No. 5;society. J. Pak. Med. Assoc., 61(3), 313-9. Hoodfar, H., Assadpour, S. (2000). The politics of population policy in the Islamic Republic of Iran. Stud. Fam. Plann., 31(1), 19-34. http://dx.doi.org/10.1111/j.1728-4465.2000.00019.x Huang, M., Hussein, H. (2004). The HIV/AIDS epidemic country paper:
Ion sequencing platforms for determining HIV-1 coreceptor useSt hanie Raymond1,2,3, Florence Nicot3, Nicolas Jeanne3, Olivier Delfour3, Romain Carcenac3, Caroline Lefebvre3, Michelle Cazabat1,3, Karine Saun?,2,3, Pierre Delobel1,2,4 Jacques Izopet1,2,The coreceptor used by HIV-1 must be determined before a CCR5 antagonist, part of the arsenal of antiretroviral drugs, is prescribed because viruse
Been fully validated for use in clinical practice. The Illumina NGS technology is used in many laboratories but its ability to predict HIV-1 tropism has not been evaluated while the 454 GS-Junior (Roche) is used for routine diagnosis. The genotypic prediction of HIV-1 tropism is based on sequencing the V3 region and interpreting the results with an appropriate algorithm. We compared the performanc