Are located in a homopolymeric region. We evaluated the sensitivity of the NGS for quantifying CXCR4-using variants with artificial mixtures of pure X4 (CHS02) and R5 (CHS11) virus clones. The MiSeq NGS detected 0.5 of X4 viruses in 2/3 mixtures and 1 in 3/3 mixtures. The 454 GS-Junior NGS detected 1 of X4 viruses in 2/3 mixtures and 5 of X4 viruses in 3/3 mixtures (Table 1). Linear regression
Use, F-31300, France. 3CHU de Toulouse, H ital Purpan, Laboratoire de Virologie, Toulouse, F-31300, France. 4CHU de Toulouse, H ital Purpan, Service des Maladies Infectieuses et Tropicales, Toulouse, F-31300, France. Correspondence and requests for materials should be addressed to S.R. (email: raymond.s@chu-toulouse.fr)Scientific RepoRts | 7:42215 | DOI: 10.1038/srepResultswww.nature.com/scientifi
Use, F-31300, France. 3CHU de Toulouse, H ital Purpan, Laboratoire de Virologie, Toulouse, F-31300, France. 4CHU de Toulouse, H ital Purpan, Service des Maladies Infectieuses et Tropicales, Toulouse, F-31300, France. Correspondence and requests for materials should be addressed to S.R. (email: raymond.s@chu-toulouse.fr)Scientific RepoRts | 7:42215 | DOI: 10.1038/srepResultswww.nature.com/scientifi
Use, F-31300, France. 3CHU de Toulouse, H ital Purpan, Laboratoire de Virologie, Toulouse, F-31300, France. 4CHU de Toulouse, H ital Purpan, Service des Maladies Infectieuses et Tropicales, Toulouse, F-31300, France. Correspondence and requests for materials should be addressed to S.R. (email: raymond.s@chu-toulouse.fr)Scientific RepoRts | 7:42215 | DOI: 10.1038/srepResultswww.nature.com/scientifi
Been fully validated for use in clinical practice. The Illumina NGS technology is used in many laboratories but its ability to predict HIV-1 tropism has not been evaluated while the 454 GS-Junior (Roche) is used for routine diagnosis. The genotypic prediction of HIV-1 tropism is based on sequencing the V3 region and interpreting the results with an appropriate algorithm. We compared the performanc
Recombinant virus phenotypic entry assay.Sensitivity of deep sequencing for detecting minor CXCR4-using variants. We determined the sensitivity threshold of NGS by calculating the error rate, either globally or for each position of V3. The mean frequency of V3 variant artifacts determined after analysis of 20 virus clones with the Illumina was 0.078 [exact Poisson 99 confidence interval (CI), 0.
E: implications for the Pakistaniwww.ccsenet.org/gjhsGlobal Journal of Health ScienceVol. 5, No. 5;society. J. Pak. Med. Assoc., 61(3), 313-9. Hoodfar, H., Assadpour, S. (2000). The politics of population policy in the Islamic Republic of Iran. Stud. Fam. Plann., 31(1), 19-34. http://dx.doi.org/10.1111/j.1728-4465.2000.00019.x Huang, M., Hussein, H. (2004). The HIV/AIDS epidemic country paper:
E: implications for the Pakistaniwww.ccsenet.org/gjhsGlobal Journal of Health ScienceVol. 5, No. 5;society. J. Pak. Med. Assoc., 61(3), 313-9. Hoodfar, H., Assadpour, S. (2000). The politics of population policy in the Islamic Republic of Iran. Stud. Fam. Plann., 31(1), 19-34. http://dx.doi.org/10.1111/j.1728-4465.2000.00019.x Huang, M., Hussein, H. (2004). The HIV/AIDS epidemic country paper: