S) and cells were lysed (1 Triton X-100 for 45 min at 37 ). Cell lysates were harvested and centrifuged at 1,800 rpm for 5 min. The amount of HIV-p24 antigen associated to cell lysates was determined using the HIV-1 p24 core profile ELISA. HIV-1 attachment on MDDC to assess the attachment of HIV-1 to MDDC, the cells were washed 2 times after 6 days of differentiation and seeded into 96-well cultu
Or media alone were added in duplicate wells, and the TER was measured at 30 min and 2, 4, 9, and 24 h. The epithelial resistance was expressed as follows :epithelial resistance = (/cm2) - the resistance of transwells without cells.HIV cell-free particles transcytosis the epithelial cell line HEC-1A was grown as a tight polarized monolayer on a permeable support of 0.4- -porediameter polycarbonate
Or media alone were added in duplicate wells, and the TER was measured at 30 min and 2, 4, 9, and 24 h. The epithelial resistance was expressed as follows :epithelial resistance = (/cm2) - the resistance of transwells without cells.HIV cell-free particles transcytosis the epithelial cell line HEC-1A was grown as a tight polarized monolayer on a permeable support of 0.4- -porediameter polycarbonate
L side of the transwell, and epithelial integrity was measured at 30 min and 2, 4, 9, and 24 h by using the Minicell-ERS instrument. The data shown are the averages from duplicate wells ?1 standard error of the mean after product addition.Page 4 of(page number not for citation purposes)Journal of Translational Medicine 2007, 5:http://www.translational-medicine.com/content/5/1/p24 (pg/ml)of the pla
The attachment phase which is (or are) (i) not an HIV-interacting GAG, as indicated by our observations in the presence of X4-tropic viruses, (ii) not C-type lectin(s), considering the absenceDiscussionThe plan lectins HHA and GNA constitute two promising microbicide molecules of great interest, capable to efficiently inhibit the infection of T lymphocytes and PBMC by a broad range of HIV strains
The attachment phase which is (or are) (i) not an HIV-interacting GAG, as indicated by our observations in the presence of X4-tropic viruses, (ii) not C-type lectin(s), considering the absenceDiscussionThe plan lectins HHA and GNA constitute two promising microbicide molecules of great interest, capable to efficiently inhibit the infection of T lymphocytes and PBMC by a broad range of HIV strains
Of plant lectins on HIV-1 transcytosis through a tight epithelial cells monolayer To evaluate the capacity of each plant lectin to inhibit in vitro HIV-1 transcytosis through genital epithelial cells, such as HEC-1A cells, we used a dual-chamber model in which the apical chamber consisted of a confluent monolayer of HEC-1A cells, and the basal chamber contained fresh medium. Cell-free virus (HIV-1
Of plant lectins on HIV-1 transcytosis through a tight epithelial cells monolayer To evaluate the capacity of each plant lectin to inhibit in vitro HIV-1 transcytosis through genital epithelial cells, such as HEC-1A cells, we used a dual-chamber model in which the apical chamber consisted of a confluent monolayer of HEC-1A cells, and the basal chamber contained fresh medium. Cell-free virus (HIV-1