Affine-registration of the brain image to standard space. This process defines the volumetric scaling factor used to normalize brain volume. To avoid introducing error from blurring associated with registration, tissue segmentation is performed on the original (non-registered) MPRAGE images and volumes are then scaled by the scaling factor to derive the normalized measurements. Freesurfer, Fischl
Nclusion criteria included: plasma HIV RNA levels (plasma viral load) 350 cells/mm3; and clinically healthy with all laboratory values grade
Nclusion criteria included: plasma HIV RNA levels (plasma viral load) 350 cells/mm3; and clinically healthy with all laboratory values grade
Nclusion criteria included: plasma HIV RNA levels (plasma viral load) 350 cells/mm3; and clinically healthy with all laboratory values grade
Nclusion criteria included: plasma HIV RNA levels (plasma viral load) 350 cells/mm3; and clinically healthy with all laboratory values grade
Expressed by the candidate vaccines were derived from an HIV-1 isolate from a vertically HIV-1-infected infant designated as C58A1 (subtype B). The env gene contained the immunodominant portion of gp41. The tat, rev, nef and reverse transcriptase (RT) genes were modified to render the proteins non-functional. These genes were inserted into two MVA and two FPV vectors; one of each pair containing e
Ing highly conserved regions of the HIV genome (Gag and Pol). PBMC were stimulated with selected pools of OLP, and live vaccinia virus as described [18]. Anti-CD107 (FITCconjugated) was added with peptide in the presence of Brefeldin A and monensin (BD Pharmingen). Following a 5-hour incubation, cells were fixed and incubated with a mixture of antibodies specific for: CD14/19 (Alexa700); CD3 (APC-
Ing highly conserved regions of the HIV genome (Gag and Pol). PBMC were stimulated with selected pools of OLP, and live vaccinia virus as described [18]. Anti-CD107 (FITCconjugated) was added with peptide in the presence of Brefeldin A and monensin (BD Pharmingen). Following a 5-hour incubation, cells were fixed and incubated with a mixture of antibodies specific for: CD14/19 (Alexa700); CD3 (APC-