Id onset of intestinal epithelial barrier dysfunction in primary human immunodeficiency virus infection is driven by an imbalance between immune response and mucosal repair and regeneration. J Virol 82: 538?45. 30. Palmer S, Wiegand AP, Maldarelli F, Bazmi H, Mican JM, et al. (2003) New real-time reverse transcriptase-initiated PCR assay with single-copy sensitivity for human immunodeficiency viru
Ndings consistent with the new onset of myopericarditis, a standardized, protocol-defined, cardiac evaluation was completed. 2.3. Clinical laboratory evaluations Complete blood counts, blood chemistries and tests to detect myopericarditis were performed in clinical laboratories at each study site. CD4 and CD8 T-cell counts, and the activation states of CD8 T-cells (HLA-DR and CD38 expression), wer
Ing highly conserved regions of the HIV genome (Gag and Pol). PBMC were stimulated with selected pools of OLP, and live vaccinia virus as described [18]. Anti-CD107 (FITCconjugated) was added with peptide in the presence of Brefeldin A and monensin (BD Pharmingen). Following a 5-hour incubation, cells were fixed and incubated with a mixture of antibodies specific for: CD14/19 (Alexa700); CD3 (APC-
Ing highly conserved regions of the HIV genome (Gag and Pol). PBMC were stimulated with selected pools of OLP, and live vaccinia virus as described [18]. Anti-CD107 (FITCconjugated) was added with peptide in the presence of Brefeldin A and monensin (BD Pharmingen). Following a 5-hour incubation, cells were fixed and incubated with a mixture of antibodies specific for: CD14/19 (Alexa700); CD3 (APC-
Ing highly conserved regions of the HIV genome (Gag and Pol). PBMC were stimulated with selected pools of OLP, and live vaccinia virus as described [18]. Anti-CD107 (FITCconjugated) was added with peptide in the presence of Brefeldin A and monensin (BD Pharmingen). Following a 5-hour incubation, cells were fixed and incubated with a mixture of antibodies specific for: CD14/19 (Alexa700); CD3 (APC-
Nclusion criteria included: plasma HIV RNA levels (plasma viral load) 350 cells/mm3; and clinically healthy with all laboratory values grade
Nclusion criteria included: plasma HIV RNA levels (plasma viral load) 350 cells/mm3; and clinically healthy with all laboratory values grade
Nclusion criteria included: plasma HIV RNA levels (plasma viral load) 350 cells/mm3; and clinically healthy with all laboratory values grade