Id onset of intestinal epithelial barrier dysfunction in primary human immunodeficiency virus infection is driven by an imbalance between immune response and mucosal repair and regeneration. J Virol 82: 538?45. 30. Palmer S, Wiegand AP, Maldarelli F, Bazmi H, Mican JM, et al. (2003) New real-time reverse transcriptase-initiated PCR assay with single-copy sensitivity for human immunodeficiency viru
Ndings consistent with the new onset of myopericarditis, a standardized, protocol-defined, cardiac evaluation was completed. 2.3. Clinical laboratory evaluations Complete blood counts, blood chemistries and tests to detect myopericarditis were performed in clinical laboratories at each study site. CD4 and CD8 T-cell counts, and the activation states of CD8 T-cells (HLA-DR and CD38 expression), wer
Ing highly conserved regions of the HIV genome (Gag and Pol). PBMC were stimulated with selected pools of OLP, and live vaccinia virus as described [18]. Anti-CD107 (FITCconjugated) was added with peptide in the presence of Brefeldin A and monensin (BD Pharmingen). Following a 5-hour incubation, cells were fixed and incubated with a mixture of antibodies specific for: CD14/19 (Alexa700); CD3 (APC-
Ing highly conserved regions of the HIV genome (Gag and Pol). PBMC were stimulated with selected pools of OLP, and live vaccinia virus as described [18]. Anti-CD107 (FITCconjugated) was added with peptide in the presence of Brefeldin A and monensin (BD Pharmingen). Following a 5-hour incubation, cells were fixed and incubated with a mixture of antibodies specific for: CD14/19 (Alexa700); CD3 (APC-
Ing highly conserved regions of the HIV genome (Gag and Pol). PBMC were stimulated with selected pools of OLP, and live vaccinia virus as described [18]. Anti-CD107 (FITCconjugated) was added with peptide in the presence of Brefeldin A and monensin (BD Pharmingen). Following a 5-hour incubation, cells were fixed and incubated with a mixture of antibodies specific for: CD14/19 (Alexa700); CD3 (APC-
Expressed by the candidate vaccines were derived from an HIV-1 isolate from a vertically HIV-1-infected infant designated as C58A1 (subtype B). The env gene contained the immunodominant portion of gp41. The tat, rev, nef and reverse transcriptase (RT) genes were modified to render the proteins non-functional. These genes were inserted into two MVA and two FPV vectors; one of each pair containing e
Nclusion criteria included: plasma HIV RNA levels (plasma viral load) 350 cells/mm3; and clinically healthy with all laboratory values grade
Nclusion criteria included: plasma HIV RNA levels (plasma viral load) 350 cells/mm3; and clinically healthy with all laboratory values grade