Al. [14] recently showed that usage of LF-LAM for hospitalized patients with low CD4+ cell count in South Africa was considered highly cost-effective compared with smearmicroscopy alone. The cost-effectiveness of incorporating LF-LAM testing as part of diagnostic algorithms with or without Xpert for a broader population of HIV-infected individuals, including outpatients and those with less immunos
E trade-offs with regards to costs and performance in people living with HIV. The cost-effectiveness of novel diagnostic algorithms that incorporate these new tools alone or in combination with each other and with conventional diagnostic modalities is unknown. Prior studies have suggested that Xpert replacement of smear-microscopy is cost-effective for diagnosis of pulmonary TB in the general popu
E trade-offs with regards to costs and performance in people living with HIV. The cost-effectiveness of novel diagnostic algorithms that incorporate these new tools alone or in combination with each other and with conventional diagnostic modalities is unknown. Prior studies have suggested that Xpert replacement of smear-microscopy is cost-effective for diagnosis of pulmonary TB in the general popu
Pathways such as macropinocytosis which is the canonical pathway for EBOV entry. Our data indicate that MBL also mediates internalization of virus via macropinocytosis but suggests that MBL-mediated uptake preferentially utilizes microtubules compared with the canonical EBOV pathway which is dependent on both microtubules and actin. doi:10.1371/journal.pone.0060838.gcirculating C4 [88], we specula
Pathways such as macropinocytosis which is the canonical pathway for EBOV entry. Our data indicate that MBL also mediates internalization of virus via macropinocytosis but suggests that MBL-mediated uptake preferentially utilizes microtubules compared with the canonical EBOV pathway which is dependent on both microtubules and actin. doi:10.1371/journal.pone.0060838.gcirculating C4 [88], we specula
Ge activity .500 U/mL (13/21 vs 1/ 14, p,0.005) compared with MBL2 O/O or O/A haplotypes (O refers to B,C or D alleles). (EPS) Figure S3 Endoglycosidases cleave N-linked glycans in HIV-EBOV GP. We preincubated HIV-EBOZ GP virion-like particles (12,000 pg/ml) with PNGase F or endo H (10,000 U/ml each) diluted in DMEM or with DMEM alone for 1 hour at 37uC. Viruses then underwent gel electrophoresis;
Exposed to autoradiography film. High-mannose moieties were preserved only in untreated virus confirming that PNGase F and endo H effectively cleaved high-mannose residues on GPs of HIV-EBOV virion-like particles. (EPS) Figure S4 Thermolysin treatment of HIV-EBOV GP abrogates enhancement of infection by rhMBL in a thermolysin-concentration dependent manner. We preincubated HIV-EBOV GP virion-like
Exposed to autoradiography film. High-mannose moieties were preserved only in untreated virus confirming that PNGase F and endo H effectively cleaved high-mannose residues on GPs of HIV-EBOV virion-like particles. (EPS) Figure S4 Thermolysin treatment of HIV-EBOV GP abrogates enhancement of infection by rhMBL in a thermolysin-concentration dependent manner. We preincubated HIV-EBOV GP virion-like