Hat both the mutant and wild-type Pol bound to monoubiquitinated PCNA in BPDE-treated cells, indicating that at least one of the two ubiquitin-binding domains (designated UBZs) was involved in the binding. This result eliminates a trivial explanation that the reduced REV1-binding activity of the Pol mutant protein is due to failure in protein folding. Thus, we conclude that the interaction with RE
Ene contexts. For Type II molecules, variability of interactants found in individual experiments for such proteins is overcome by analyzing large single IP/MS studies like the one presented here. ItCell. Author manuscript; available in PMC 2012 May 27.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptMalovannaya et al.Pagethen becomes clear that a protein that biochemically
Ene contexts. For Type II molecules, variability of interactants found in individual experiments for such proteins is overcome by analyzing large single IP/MS studies like the one presented here. ItCell. Author manuscript; available in PMC 2012 May 27.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptMalovannaya et al.Pagethen becomes clear that a protein that biochemically
The protein among evolutionarily distant species and highlights Dictyostelium as a valid experimental system to address the function of this gene. Dictyostelium Vmp1 is an endoplasmic reticulum protein necessary for the integrity of this organelle. Cells deficient in Vmp1 display pleiotropic defects in the secretory pathway and organelle biogenesis. The contractile vacuole, which is necessary to s
The protein among evolutionarily distant species and highlights Dictyostelium as a valid experimental system to address the function of this gene. Dictyostelium Vmp1 is an endoplasmic reticulum protein necessary for the integrity of this organelle. Cells deficient in Vmp1 display pleiotropic defects in the secretory pathway and organelle biogenesis. The contractile vacuole, which is necessary to s
Mic blasts at diagnosis. The values of the AAI profile increased again at relapse, indicating apoptosis-resistance (11). Based on these unexpected findings, we hypothesized that expression of apoptosis-related proteins in AML blasts, and possibly also in bystander cells in the bone marrow, is regulated by extracellular factors present in the AML microenvironment. Tumor cell communication with its
Mic blasts at diagnosis. The values of the AAI profile increased again at relapse, indicating apoptosis-resistance (11). Based on these unexpected findings, we hypothesized that expression of apoptosis-related proteins in AML blasts, and possibly also in bystander cells in the bone marrow, is regulated by extracellular factors present in the AML microenvironment. Tumor cell communication with its
Positioning these proteins at the site of the developing exosporium (57, 58, 65?7). No such localization domain is present in the BxpB sequence. Therefore, my colleagues and I have proposed a model whereby BxpB is delivered to the spore by virtue of forming a complex with BclA and possibly, to a lesser extent, with other exosporium-targeting motif sequences (58). In keeping with this, we observed