Culate, as a function of the serum antibody concentration, the probability of a trivalent MPER being disabled by polyreactive mAbs. A question we also consider is how the probability of disabling a single epitope is related to the probability of blocking membrane fusion of HIV-1 and target cell when, at the start of the pre-hairpin intermediate stage, N epitopes bridge the two membranes. We presen
Culate, as a function of the serum antibody concentration, the probability of a trivalent MPER being disabled by polyreactive mAbs. A question we also consider is how the probability of disabling a single epitope is related to the probability of blocking membrane fusion of HIV-1 and target cell when, at the start of the pre-hairpin intermediate stage, N epitopes bridge the two membranes. We presen
L to fit neutralization experiments where neutralization is achieved with either 4E10 or 2F5 IgG or Fab. Our analysis of these experiments indicates that at the start of the pre-hairpin intermediate only a few gp41 trimers are involved in bridging the virion and target cell. As the bonds break sequentially those that remain come under additional strain and are disabled more easily.membrane fusion
Chiometry of entry. For example, if in Eq. (13) we have that p1 p2 1 and p3 v1, then T 3. If the virion and target cell are attached by three trimers, if one of those trimers is disable, the two remaining trimers will spontaneously disable. The stoichiometry of entry has yet to be rigorously determined, but it is most probably greater than one [32]. To analyze neutralization experiments we treat
Chiometry of entry. For example, if in Eq. (13) we have that p1 p2 1 and p3 v1, then T 3. If the virion and target cell are attached by three trimers, if one of those trimers is disable, the two remaining trimers will spontaneously disable. The stoichiometry of entry has yet to be rigorously determined, but it is most probably greater than one [32]. To analyze neutralization experiments we treat
Aining undefined nucleotides (`N's) were filtered out. This can occur when the signals of nucleotides incorporated in a cluster are much lower than those of surrounding clusters. Only sequences with an average phred equivalent quality score of >Q30 were retained.sus sequence (GenBank accession no. AY426110.1) and processed using an in-house automated data cleaning strategy9. Non-functional V3 sequ
Been fully validated for use in clinical practice. The Illumina NGS technology is used in many laboratories but its ability to predict HIV-1 tropism has not been evaluated while the 454 GS-Junior (Roche) is used for routine diagnosis. The genotypic prediction of HIV-1 tropism is based on sequencing the V3 region and interpreting the results with an appropriate algorithm. We compared the performanc
Been fully validated for use in clinical practice. The Illumina NGS technology is used in many laboratories but its ability to predict HIV-1 tropism has not been evaluated while the 454 GS-Junior (Roche) is used for routine diagnosis. The genotypic prediction of HIV-1 tropism is based on sequencing the V3 region and interpreting the results with an appropriate algorithm. We compared the performanc