Inhibitor (which is involved in clathrin- and caveolin-mediated endocytosis) in 5 MBL-deficient serum in the absence or presence of rhMBL at 37uC for 1 hour. We then infected cells with HIV-EBOV-GP virion-like particles (1200 pg p24/100 ml). Percentages of infected cells are relative to DMSO controls (a methanol control was used for monensin). Luciferase values were adjusted for cell viability us
Is reduced [8]. By contrast, newly emerging urine tests for LAM may enhance diagnostic algorithms by offering additional diagnostic yield for HIV-associated TB [2,9?1]. Urinary LAM detection offers the benefit of evaluating non-respiratory samples and has additive value when combined with sputum smear microscopy in HIV-infected individuals with signs/symptoms of TB [2,9,10,12]. In HIV-positive adu
Is reduced [8]. By contrast, newly emerging urine tests for LAM may enhance diagnostic algorithms by offering additional diagnostic yield for HIV-associated TB [2,9?1]. Urinary LAM detection offers the benefit of evaluating non-respiratory samples and has additive value when combined with sputum smear microscopy in HIV-infected individuals with signs/symptoms of TB [2,9,10,12]. In HIV-positive adu
In part, the evolutionary selection of MBL mutant haplotypes that encode low or intermediate MBL serum levels in the majority of humans.Supporting InformationFigure S1 RhMBL enhances HIV-EBOV GP infection in a calcium-dependent manner. We preincubated HIVEBOV-GP virion-like particles with rhMBL in 5 MBL-deficient serum and Veronal-buffered saline with or without calcium supplementation. *, ** and
In part, the evolutionary selection of MBL mutant haplotypes that encode low or intermediate MBL serum levels in the majority of humans.Supporting InformationFigure S1 RhMBL enhances HIV-EBOV GP infection in a calcium-dependent manner. We preincubated HIVEBOV-GP virion-like particles with rhMBL in 5 MBL-deficient serum and Veronal-buffered saline with or without calcium supplementation. *, ** and
Ge activity .500 U/mL (13/21 vs 1/ 14, p,0.005) compared with MBL2 O/O or O/A haplotypes (O refers to B,C or D alleles). (EPS) Figure S3 Endoglycosidases cleave N-linked glycans in HIV-EBOV GP. We preincubated HIV-EBOZ GP virion-like particles (12,000 pg/ml) with PNGase F or endo H (10,000 U/ml each) diluted in DMEM or with DMEM alone for 1 hour at 37uC. Viruses then underwent gel electrophoresis;
Ge activity .500 U/mL (13/21 vs 1/ 14, p,0.005) compared with MBL2 O/O or O/A haplotypes (O refers to B,C or D alleles). (EPS) Figure S3 Endoglycosidases cleave N-linked glycans in HIV-EBOV GP. We preincubated HIV-EBOZ GP virion-like particles (12,000 pg/ml) with PNGase F or endo H (10,000 U/ml each) diluted in DMEM or with DMEM alone for 1 hour at 37uC. Viruses then underwent gel electrophoresis;
Exposed to autoradiography film. High-mannose moieties were preserved only in untreated virus confirming that PNGase F and endo H effectively cleaved high-mannose residues on GPs of HIV-EBOV virion-like particles. (EPS) Figure S4 Thermolysin treatment of HIV-EBOV GP abrogates enhancement of infection by rhMBL in a thermolysin-concentration dependent manner. We preincubated HIV-EBOV GP virion-like