Dons at each V3 position among the 20 virus clones (Fig. 1). We then attributed a weighted error rate to each position and used these rates to construct a sensitivity threshold matrix for each position of V3 to retain a minor virus variant harboring point mutations as authentic for a given number of reads with P
He expected values and the values obtained with both MiSeq ( = 1, p
Ion sequencing platforms for determining HIV-1 coreceptor useSt hanie Raymond1,2,3, Florence Nicot3, Nicolas Jeanne3, Olivier Delfour3, Romain Carcenac3, Caroline Lefebvre3, Michelle Cazabat1,3, Karine Saun?,2,3, Pierre Delobel1,2,4 Jacques Izopet1,2,The coreceptor used by HIV-1 must be determined before a CCR5 antagonist, part of the arsenal of antiretroviral drugs, is prescribed because viruse
Dons at each V3 position among the 20 virus clones (Fig. 1). We then attributed a weighted error rate to each position and used these rates to construct a sensitivity threshold matrix for each position of V3 to retain a minor virus variant harboring point mutations as authentic for a given number of reads with P
F a CCR5-antagonist-based treatment4,5. Deep sequencing techniques can detect minor variants, especially ones that use CXCR46?. The 454 GS-Junior sequencing platform (Roche) can reliably predict HIV-1 tropism9?1. The Ion Torrent Personal Genome Machine has also been validated for tropism determination12. Another study evaluated the Illumina platform for determining HIV-1 drug resistance and HIV-1
Anti-HIV therapeutic levels in the CNS.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptDISCUSSIONEffective CNS delivery of many drugs, especially hydrophilic and negatively charged molecules, still remains an unsolved dilemma. Progress of nanotechnology and development of novel particulate drug delivery systems resulted in the extensive search for drug nanoformulations th
Recombinant virus phenotypic entry assay.Sensitivity of deep sequencing for detecting minor CXCR4-using variants. We determined the sensitivity threshold of NGS by calculating the error rate, either globally or for each position of V3. The mean frequency of V3 variant artifacts determined after analysis of 20 virus clones with the Illumina was 0.078 [exact Poisson 99 confidence interval (CI), 0.
Anti-HIV therapeutic levels in the CNS.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptDISCUSSIONEffective CNS delivery of many drugs, especially hydrophilic and negatively charged molecules, still remains an unsolved dilemma. Progress of nanotechnology and development of novel particulate drug delivery systems resulted in the extensive search for drug nanoformulations th