S the same as sensitivity to dilution or drugs). The mistaken gestalt that sometimes comes to mind is that a stable microtubule is necessarily stiffer or less mobile than a labile microtubule. Some proteins that stabilize microtubules could also make them stiffer or less mobile, but this is not always true. For example, a microtubule could be stable but not as stiff as a microtubule that is labile
S the same as sensitivity to dilution or drugs). The mistaken gestalt that sometimes comes to mind is that a stable microtubule is necessarily stiffer or less mobile than a labile microtubule. Some proteins that stabilize microtubules could also make them stiffer or less mobile, but this is not always true. For example, a microtubule could be stable but not as stiff as a microtubule that is labile
Ion of other organs (44). MMPs, especially S100A8/A9, induce inflammation-related pathways in the tumor microenvironment, which are necessary for premetastatic niche formation (44, 45). One goal of our study was to investigate the potential use of differentially released proteins as CRC diagnostic biomarkers in serum. Although these biomarkers were obtained from comparing metastatic versus nonmeta
Ion of other organs (44). MMPs, especially S100A8/A9, induce inflammation-related pathways in the tumor microenvironment, which are necessary for premetastatic niche formation (44, 45). One goal of our study was to investigate the potential use of differentially released proteins as CRC diagnostic biomarkers in serum. Although these biomarkers were obtained from comparing metastatic versus nonmeta
Affect quantification accuracy in some cell types, we investigated arginine to proline conversion in KM12 cells. By adding heavy proline as a variable modification, less than 2 of proline-containing peptides were heavy labeled in KM12 cells, which has been reported not to affect significantly SILAC quantification (25). Western Blot--For Western blot, conditioned media from KM12C and KM12SM CRC ce
Ogenized and centrifuged at 1000 g, and the supernatant was removed. For measurement of forskolinstimulated cAMP in lysate, we added 100 ?00 g of tissue to incubation buffer containing 50 mM ATP, 30 M IBMX, and 5 mM creatine phosphate with and without forskolin (50 M). We incubated the mixture for 10 min at 37 in a water bath and stopped the reaction by adding 5 TCA, which was subsequently extra
S containing healthy dinoflagellates remained free of either marker. Studies of the anemone A. pulchella have generated further evidence, by tracking the location of Rab GTPases, that phagosomal maturation and endosomal trafficking (described in Appendix 1) are altered by the presence of Symbiodinium cells in phagosomes (see Appendix 1). First, cnidarian orthologs to human Rab5 and -7 were sequenc
S containing healthy dinoflagellates remained free of either marker. Studies of the anemone A. pulchella have generated further evidence, by tracking the location of Rab GTPases, that phagosomal maturation and endosomal trafficking (described in Appendix 1) are altered by the presence of Symbiodinium cells in phagosomes (see Appendix 1). First, cnidarian orthologs to human Rab5 and -7 were sequenc