E compared this rapid algorithm with current TB diagnostic approaches, which rely upon sputum examination by smear-microscopy or Xpert alone [15].NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptMethodsEthics statement The study was approved by the institutional review board (IRB) at the Johns Hopkins University School of Medicine (Baltimore, Maryland, USA), as well as in
E compared this rapid algorithm with current TB diagnostic approaches, which rely upon sputum examination by smear-microscopy or Xpert alone [15].NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptMethodsEthics statement The study was approved by the institutional review board (IRB) at the Johns Hopkins University School of Medicine (Baltimore, Maryland, USA), as well as in
E compared this rapid algorithm with current TB diagnostic approaches, which rely upon sputum examination by smear-microscopy or Xpert alone [15].NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptMethodsEthics statement The study was approved by the institutional review board (IRB) at the Johns Hopkins University School of Medicine (Baltimore, Maryland, USA), as well as in
E compared this rapid algorithm with current TB diagnostic approaches, which rely upon sputum examination by smear-microscopy or Xpert alone [15].NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptMethodsEthics statement The study was approved by the institutional review board (IRB) at the Johns Hopkins University School of Medicine (Baltimore, Maryland, USA), as well as in
In part, the evolutionary selection of MBL mutant haplotypes that encode low or intermediate MBL serum levels in the majority of humans.Supporting InformationFigure S1 RhMBL enhances HIV-EBOV GP infection in a calcium-dependent manner. We preincubated HIVEBOV-GP virion-like particles with rhMBL in 5 MBL-deficient serum and Veronal-buffered saline with or without calcium supplementation. *, ** and
On of fetal calf or fetal bovine serum typically used for EBOV infectivity assays [17,18,44,45,67]. Given that C4 gene copy number variations may lead to a proportionate reduction ofLectin-Dependent Enhancement of Ebola Virusesiological doses of MBL products or blood products with high MBL concentrations to individuals in the setting of infectious diseases and relative hypocomplementemia may be de
Exposed to autoradiography film. High-mannose moieties were preserved only in untreated virus confirming that PNGase F and endo H effectively cleaved high-mannose residues on GPs of HIV-EBOV virion-like particles. (EPS) Figure S4 Thermolysin treatment of HIV-EBOV GP abrogates enhancement of infection by rhMBL in a thermolysin-concentration dependent manner. We preincubated HIV-EBOV GP virion-like
Exposed to autoradiography film. High-mannose moieties were preserved only in untreated virus confirming that PNGase F and endo H effectively cleaved high-mannose residues on GPs of HIV-EBOV virion-like particles. (EPS) Figure S4 Thermolysin treatment of HIV-EBOV GP abrogates enhancement of infection by rhMBL in a thermolysin-concentration dependent manner. We preincubated HIV-EBOV GP virion-like