Bit Ability ofepithelial cells attachment of HIV-1 cell-free parAbility of lectins to inhibit attachment of HIV-1 cell-free particles on epithelial cells. HEC-1A cells were co-incubated with non-toxic concentrations of each lectins or mannan (100 /ml) and 5 ng of HIV-1Ba-L (A) or HIV-1NDK (B) virus were added for 1 h. Incubation with mannan was used as control. The quantity of attached virus was
Glycans (GAG) heparan sulfates, we assessed the role of plant lectins on the attachment of X4-tropic viruses, known to interact very efficiently with heparan sulfate [11] and data not shown]. Thus, cells were incubated with HIV-1NDK (Fig. 2B) in the presence or absence of different concentrations of HHA and GNA. As depicted in Figure 2B, none of the plant lectins, whatever the concentration, inhib
Glycans (GAG) heparan sulfates, we assessed the role of plant lectins on the attachment of X4-tropic viruses, known to interact very efficiently with heparan sulfate [11] and data not shown]. Thus, cells were incubated with HIV-1NDK (Fig. 2B) in the presence or absence of different concentrations of HHA and GNA. As depicted in Figure 2B, none of the plant lectins, whatever the concentration, inhib
Of attachment on MDDC resulted in subsequent inhibition of HIV-1 transmission to autologous T lymphocytes. MDDC were incubated with HIV-1Ba-L in the presence of increasing doses of each plant lectin. Free viral particles and plant lectins were removed and autologous PBL were added. The pretreatment of MDDC by each plant lectin resulted in a partially reduced infection of autologous PBL by HIV-1 (F
Nan (100 /ml) for 3 h at 37 in a 5 CO2 atmosphere. Cells were washed four times and autologous stimulated T cells were added onto HIV-exposed MDDC at a DC:T-cell ratio of 1:5. Each sample was performed in triplicate. Culture supernatants were harvested every 3 days and fresh medium was added. Supernatants were inactivated with 1 Triton X-100 and frozen at -20 . The viral production by T lympho
Er. Cells were incubated for 1 h at 37 . HIV-1 transcytosis was assessed by detecting the presence of p24 antigen (HIV-1 core profile ELISA) in the basolateral chamber of the transwell. The inhibition of transcytosis was expressed as the percentage of p24 antigen recovered in the basolateral chamber in the presence of each plant lectin by comparison to that recovered without the plant lectins. HIV
Compared with those of standard OD490 versus cell number curves generated for each cell type. The percentage survival was calculated using the formula :Epithelial monolayer integrity the effect of the plant lectins on their ability to maintain an intact epithelium was determined by measuring transepithelial resistance (TER). HEC-1A cells were grown as a tight polarized monolayer on a permeable sup
Compared with those of standard OD490 versus cell number curves generated for each cell type. The percentage survival was calculated using the formula :Epithelial monolayer integrity the effect of the plant lectins on their ability to maintain an intact epithelium was determined by measuring transepithelial resistance (TER). HEC-1A cells were grown as a tight polarized monolayer on a permeable sup