64188 - High DR Web Submission Directory - S) and cells were lysed (1 Triton X-100 for 45 min at 37 ). Cell http://64188.org/story.php?title=s-and-cells-were-lysed-1-triton-x-100-for-45-min-at-37--cell-3 S) and cells were lysed (1 Triton X-100 for 45 min at 37 ). Cell lysates were harvested and centrifuged at 1,800 rpm for 5 min. The amount of HIV-p24 antigen associated to cell lysates was determined using the HIV-1 p24 core profile ELISA. HIV-1 attachment on MDDC to assess the attachment of HIV-1 to MDDC, the cells were washed 2 times after 6 days of differentiation and seeded into 96-well cultu Tue, 23 May 2023 20:06:29 UTC en