http://64188.org/story.php?title=re-in-the-dark-for-30-minutes-proteins-were-then-digested-with Re in the dark for 30 minutes. Proteins were then digested with modified porcine trypsin (Promega Gold, Promega, Madison, WI) at a protein to trypsin ratio of 50:1 overnight at 37 . Proteolysis was quenched and Rapigest was cleaved with the addition of trifluoroacetic acid and acetonitrile to 1 and 2 (v/v), respectively. The samples were each spiked with 50 fmol yeast alcohol dehydrogenase (Mas Mon, 16 Jan 2023 03:58:38 UTC en