http://64188.org/story.php?title=proteomes-exhibit-a-2-net-charge-at-ph-2-7-and-can-be-separated Proteomes exhibit a 2 net charge at pH 2.7 and can be separated from other peptides according to single charge differences using SCX chromatography (33). Because trypsin cuts poorly at Meth-R, the missed cleavages produce peptides with charge 3 at pH 2.7. Thus, most Meth-R peptides should be present in the 3 peptide-containing fractions after SCX chromatography. SCX fractions enriched for tryptic Wed, 19 Apr 2023 13:51:42 UTC en