http://64188.org/story.php?title=nd-were-processed-for-assays-or-cryopreservation-of-blood-components-within-6 Nd were processed for assays or cryopreservation of blood components within 28 hours of phlebotomy. Lymphocyte proliferative responses to whole protein antigens were measured using a membrane dye dilution methodology adapted from da Silva et al [15]. PBMC were treated with 8M PKH26, a fluorescent membrane dye, using the manufacturer's protocol (Sigma Chemical Co.), then cultured in duplicate at 2 Tue, 18 Apr 2023 15:17:32 UTC en