http://64188.org/story.php?title=harvested-and-resuspended-in-a-lysis-buffer-containing-0-4-m-sucrose-50-mm-9 Harvested and resuspended in a lysis buffer containing 0.4 M sucrose, 50 mM 3-(Nmorpholino) propanesulfonic acid, pH 7.0, 10 mM NaCl, 5 mM EDTA, and 0.5 mM PMSF. Cells were broken using a bead beater, and centrifuged with 5,000 g for 30 min at 4 to remove glass beads and insoluble cell debris. The membrane and the soluble fractions were separated as previously described with slight modifications Mon, 16 Jan 2023 12:14:30 UTC en