http://64188.org/story.php?title=ent-pharmacia-isolation-of-pbls-their-activation-with-phail-2-and Ent (Pharmacia). Isolation of PBLs, their activation with PHA/IL-2 and infection with HIV-1LAI followed by isolation of resting CD4+ T cells by negative selection using magnetic beads (Invitrogen) was performed as previously described (Contreras et al., 2007). Resting CD4+ T cells were treated with 1mM HMBA and 10 ng/ml TNF- for 24 h. Viral release in the supernatant was quantified by p24 ELISA (P Wed, 01 Feb 2023 00:45:50 UTC en