http://64188.org/story.php?title=aining-undefined-nucleotides-ns-were-filtered-out-this-can-occur Aining undefined nucleotides (`N's) were filtered out. This can occur when the signals of nucleotides incorporated in a cluster are much lower than those of surrounding clusters. Only sequences with an average phred equivalent quality score of >Q30 were retained.sus sequence (GenBank accession no. AY426110.1) and processed using an in-house automated data cleaning strategy9. Non-functional V3 sequ Tue, 14 Mar 2023 03:13:49 UTC en