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Re in the dark for 30 minutes. Proteins were then digested with modified porcine trypsin (Promega Gold, Promega, Madison, WI) at a protein to trypsin ratio of 50:1 overnight at 37 . Proteolysis was quenched and Rapigest was cleaved with the addition of trifluoroacetic acid and acetonitrile to 1 and 2 (v/v), respectively. The samples were each spiked with 50 fmol yeast alcohol dehydrogenase (Mas
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