Ost Microbe. Author manuscript; available in PMC 2014 November 03.Lenasi et al.PageWestern blotting 107 J-Lat or J-Lat shER cells were lysed in 0.8 ml of lysis buffer A (10 mM Tris-HCl [pH 7.4], 150 mM NaCl, 2 mM EDTA, 1 NP-40, 0.1 protease inhibitor) for 40 min at 4 . Proteins in the lysates were separated on SDS-PAGE electrophoresis. Western blotting was performed according to the standard pro