Ent (Pharmacia). Isolation of PBLs, their activation with PHA/IL-2 and infection with HIV-1LAI followed by isolation of resting CD4+ T cells by negative selection using magnetic beads (Invitrogen) was performed as previously described (Contreras et al., 2007). Resting CD4+ T cells were treated with 1mM HMBA and 10 ng/ml TNF- for 24 h. Viral release in the supernatant was quantified by p24 ELISA (P