Infections using high level rhMBL (10 mg/ml) in 5 serum (i.e. diluted 20-fold). However, such a high concentration of rhMBL is considered supraphysiological if adjusted for the 20-fold dilution factor of serum. Although human MBL is produced primarily in the liver, extrahepatic MBL synthesis has been found in the gastrointestinal tract, middle ear fluid, and nasopharyngeal secretions. It is possi

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Ge activity .500 U/mL (13/21 vs 1/ 14, p,0.005) compared with MBL2 O/O or O/A haplotypes (O refers to B,C or D alleles). (EPS) Figure S3 Endoglycosidases cleave N-linked glycans in HIV-EBOV GP. We preincubated HIV-EBOZ GP virion-like particles (12,000 pg/ml) with PNGase F or endo H (10,000 U/ml each) diluted in DMEM or with DMEM alone for 1 hour at 37uC. Viruses then underwent gel electrophoresis;

Es obtained with the LTR-specific primers represent a sum of transcripts originating from the host promoter and of viral transcripts, which also contain LTR sequence at their 3' ends. Therefore, the measured ratio corresponds to the equation a+b/b, where `a' is the number of host-viral chimeric transcripts and `b' the number of viral transcripts (see Supplemental text 2). First, we determined this

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Lar haplotype assays (BioTest ABC SSPtray; BioTest Diagnostics Corp, Denville, NJ). Vaccinia-specific CD8 T-cell IFN and MIP-1 production was by flow cytometry measured using stimulation with live vaccinia virus (NYCBH strain) at an MOI of 5 as described by Precopio et al [18]. ELISPOT assays were performed utilizing MabtechTM ELISpot plus Human Interferon-gamma kits (MABTECH AB, Sweden). Cryopres

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