Or media alone were added in duplicate wells, and the TER was measured at 30 min and 2, 4, 9, and 24 h. The epithelial resistance was expressed as follows :epithelial resistance = (/cm2) - the resistance of transwells without cells.HIV cell-free particles transcytosis the epithelial cell line HEC-1A was grown as a tight polarized monolayer on a permeable support of 0.4- -porediameter polycarbonate

Or media alone were added in duplicate wells, and the TER was measured at 30 min and 2, 4, 9, and 24 h. The epithelial resistance was expressed as follows :epithelial resistance = (/cm2) - the resistance of transwells without cells.HIV cell-free particles transcytosis the epithelial cell line HEC-1A was grown as a tight polarized monolayer on a permeable support of 0.4- -porediameter polycarbonate

Or media alone were added in duplicate wells, and the TER was measured at 30 min and 2, 4, 9, and 24 h. The epithelial resistance was expressed as follows :epithelial resistance = (/cm2) - the resistance of transwells without cells.HIV cell-free particles transcytosis the epithelial cell line HEC-1A was grown as a tight polarized monolayer on a permeable support of 0.4- -porediameter polycarbonate

S) and cells were lysed (1 Triton X-100 for 45 min at 37 ). Cell lysates were harvested and centrifuged at 1,800 rpm for 5 min. The amount of HIV-p24 antigen associated to cell lysates was determined using the HIV-1 p24 core profile ELISA. HIV-1 attachment on MDDC to assess the attachment of HIV-1 to MDDC, the cells were washed 2 times after 6 days of differentiation and seeded into 96-well cultu

Compared with those of standard OD490 versus cell number curves generated for each cell type. The percentage survival was calculated using the formula :Epithelial monolayer integrity the effect of the plant lectins on their ability to maintain an intact epithelium was determined by measuring transepithelial resistance (TER). HEC-1A cells were grown as a tight polarized monolayer on a permeable sup

Or media alone were added in duplicate wells, and the TER was measured at 30 min and 2, 4, 9, and 24 h. The epithelial resistance was expressed as follows :epithelial resistance = (/cm2) - the resistance of transwells without cells.HIV cell-free particles transcytosis the epithelial cell line HEC-1A was grown as a tight polarized monolayer on a permeable support of 0.4- -porediameter polycarbonate

Compared with those of standard OD490 versus cell number curves generated for each cell type. The percentage survival was calculated using the formula :Epithelial monolayer integrity the effect of the plant lectins on their ability to maintain an intact epithelium was determined by measuring transepithelial resistance (TER). HEC-1A cells were grown as a tight polarized monolayer on a permeable sup

Compared with those of standard OD490 versus cell number curves generated for each cell type. The percentage survival was calculated using the formula :Epithelial monolayer integrity the effect of the plant lectins on their ability to maintain an intact epithelium was determined by measuring transepithelial resistance (TER). HEC-1A cells were grown as a tight polarized monolayer on a permeable sup