Programme): an international survey. Lancet 2002, 360:1631?639. Rutten FH, Grobbee DE, Hoes AW: Differences between general practitioners and cardiologists in diagnosis and management of heart failure: a survey in every-day practice. Eur J Heart Fail 2003, 5:337?44. Rostagno C, Olivo G, Comeglio M, Boddi V, Banchelli M, Galanti G, et al: Prognostic value of 6-minute walk corridor test in patients

S) and cells were lysed (1 Triton X-100 for 45 min at 37 ). Cell lysates were harvested and centrifuged at 1,800 rpm for 5 min. The amount of HIV-p24 antigen associated to cell lysates was determined using the HIV-1 p24 core profile ELISA. HIV-1 attachment on MDDC to assess the attachment of HIV-1 to MDDC, the cells were washed 2 times after 6 days of differentiation and seeded into 96-well cultu

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Er. Cells were incubated for 1 h at 37 . HIV-1 transcytosis was assessed by detecting the presence of p24 antigen (HIV-1 core profile ELISA) in the basolateral chamber of the transwell. The inhibition of transcytosis was expressed as the percentage of p24 antigen recovered in the basolateral chamber in the presence of each plant lectin by comparison to that recovered without the plant lectins. HIV

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Translocator proteins in P. aeruginosa or S. typhimuriurm, does not result in loss of effector secretion control (Broms et al., 2003, Cisz et al., 2008, Kaniga et al., 1995b). Another variation of the plug model has been proposed, where a `sensor' protein has been inserted into the type III secretion channel (Blocker et al., 2008). Its export is inhibited by the type III secretion needle-tip and i