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Produced in BL21 bacteria (Stratagene) using 0.5 mM IPTG induction. Bacterial pellets were lysed in NETN buffer (50 mM Tris-HCl pH8.0, 150 mM NaCl, 0.5 NP40, 5 mM EDTA, PIs), cleared by ultracentrifugation and bound to glutathione sepharose 4B (GE Healthcare) for 1 hr at 4 . Beads were washed with NETN, and amount of GST protein was estimated by SDS-PAGE followed by Coomassie staining. GST fusion
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Ene contexts. For Type II molecules, variability of interactants found in individual experiments for such proteins is overcome by analyzing large single IP/MS studies like the one presented here. ItCell. Author manuscript; available in PMC 2012 May 27.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptMalovannaya et al.Pagethen becomes clear that a protein that biochemically
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Ries and relationships to emerge as specifically salient (bioactive molecules), even thoughRies and rela
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ME mapping file.These information incorporate unique sample identifiers, distinctive barcodeME mapping f
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Stic, nonconformist, selfreliant, selfdirected and proactive, operating using a degree ofStic, nonconfor
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Stic, nonconformist, selfreliant, selfdirected and proactive, operating using a degree ofStic, nonconfor
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Stic, nonconformist, selfreliant, selfdirected and proactive, operating having a degree ofStic, nonconfo
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