Produced in BL21 bacteria (Stratagene) using 0.5 mM IPTG induction. Bacterial pellets were lysed in NETN buffer (50 mM Tris-HCl pH8.0, 150 mM NaCl, 0.5 NP40, 5 mM EDTA, PIs), cleared by ultracentrifugation and bound to glutathione sepharose 4B (GE Healthcare) for 1 hr at 4 . Beads were washed with NETN, and amount of GST protein was estimated by SDS-PAGE followed by Coomassie staining. GST fusion

Ene contexts. For Type II molecules, variability of interactants found in individual experiments for such proteins is overcome by analyzing large single IP/MS studies like the one presented here. ItCell. Author manuscript; available in PMC 2012 May 27.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptMalovannaya et al.Pagethen becomes clear that a protein that biochemically

Ed to fully capture the perspectives of this crucial population.UnderstandingEd to fully capture the per

Ries and relationships to emerge as specifically salient (bioactive molecules), even thoughRies and rela

ME mapping file.These information incorporate unique sample identifiers, distinctive barcodeME mapping f

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Stic, nonconformist, selfreliant, selfdirected and proactive, operating using a degree ofStic, nonconfor

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