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Xpressed proteins. Functional Validation of Interactions by shRNA-Mediated Knockdown--To determine the extent to which the high confidence interactors identified by I-DIRT play a role in DENV infection, we used shRNA to silence a panel of host genes and then determined the effect on DENV infection. The genes in our panel included interactors of both NS5-GFP and NS3GFP (CAD, DHCR7), only NS5-GFP (M

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S) and cells were lysed (1 Triton X-100 for 45 min at 37 ). Cell lysates were harvested and centrifuged at 1,800 rpm for 5 min. The amount of HIV-p24 antigen associated to cell lysates was determined using the HIV-1 p24 core profile ELISA. HIV-1 attachment on MDDC to assess the attachment of HIV-1 to MDDC, the cells were washed 2 times after 6 days of differentiation and seeded into 96-well cultu

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